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目的 :探讨器官低温保存的最适 p H环境 ,改善保存器官的质量。方法 :鼠肝分别被保存在 p H6 .8,7.4,8.0 ,8.6四种改良 Euro- Collins保存液中 ,10 h后行鼠肝移植。测定肝组织能量物质三磷酸腺苷 (adenosine triphos-phate,ATP)、磷酸腺苷总量 (total adenine nucleotides,TAN )、能荷 (energy charges,EC)水平和组织超微结构的改变。结果 :保存 10 h后 ,p H 8.0组肝组织中的 ATP、TAN和 EC水平明显高于 p H 8.6和 6 .8组 (P <0 .0 1)。高于 p H 7.4组 ,但无统计学差异 (P >0 .0 1)。肝组织中 ATP、TAN、EC再灌注 1h后的恢复 p H 8.0组亦比其它组明显 (P <0 .0 1或 P <0 .0 5 )。肝脏超微结构损害 p H 8.0组亦较轻。表现为肝细胞水肿轻 ,线粒体肿胀及内质网扩张程度轻。结论 :p H 8.0的改良 Euro- Collins器官保存液能改善保存大鼠肝脏的质量 ,提高其存活能力。p H 8.0可能为低温保存器官的最适 p H环境。
Objective: To investigate the optimal p H environment for organ cryopreservation and to improve the quality of organ preservation. Methods: Rat liver was preserved in four modified Euro-Collins p6, 7.4, 8.0 and 8.6, respectively. Rat liver transplantation was performed 10 h later. The changes of liver tissue energy substance adenosine triphos-phate (ATP), total adenine nucleotides (TAN), energy charges (EC) and tissue ultrastructure were determined. Results: The levels of ATP, TAN and EC in liver tissue of p H 8.0 group were significantly higher than those of p H 8.6 and 6.8 groups (P <0.01) after 10 h of storage. Higher than the p H 7.4 group, but no significant difference (P> 0. 01). The levels of ATP, TAN and EC in liver tissue recovered 1 h after reperfusion in p H 8.0 group than those in other groups (P <0.01 or P <0.05). Liver ultrastructure damage p H 8.0 group also lighter. The performance of liver cell edema, mitochondrial swelling and endoplasmic reticulum dilatation degree is light. Conclusion: The modified Euro-Collins organ preservation solution with p H 8.0 can improve the quality of the preserved rat liver and increase its viability. p H 8.0 may be the optimal p H environment for cryogenic organ preservation.