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Objective: To isolate neural progenitors from midbrain of new born rats. Methods: Cell groups with cloning capability were obtained from midbrain of new-born rats by using serum-free and floating cell culture. Immunocytochemistry was applied to detect antigens such as BrdU, Nestin and specific markers for mature neural cells. Results: Cell groups isolated from midbrain propagated in succession and shaped ’neuro-spheres’. Nestin antigen was expressed in the course of proliferation. After differentiation, epitopes of neuron , astrocyte and oligodendrocyte were all found in the neural progenitors-derived cells, the most majority were astrocyte-epitoped cells. Conclusion: Cell groups expressing Nestin isolated from midbrain of new-born rats have properties of propagating, multi-potency differentiating and self-renewal. It is demonstrated that the cell groups are stem-like neural progenitors in the central nervous system, which are qualified for cell therapy of diseases in central nervous system.
Objective: To isolate neural progenitors from midbrain of new born rats. Methods: Cell groups with cloning capability were obtained from midbrain of new-born rats by using serum-free and floating cell culture. Immunocytochemistry was applied to detect antigens such as BrdU, Nestin Results: Cell groups isolated from midbrain propagated in succession and shaped ’neuro-spheres’. Nestin antigen was expressed in the course of proliferation. After differentiation, epitopes of neuron, astrocyte and oligodendrocyte were all found in the neural progenitors-derived cells, the most majority were astrocyte-epitoped cells. Conclusion: Cell groups expressing Nestin isolated from midbrain of new-born rats have properties of propagating, multi-potency differentiating and self-renewal. It is known as that cell groups are stem-like neural progenitors in the central nervous system, which are qualified for cell therapy of diseases in central nervous s ystem.