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用小鼠骨髓细胞(活体法)和人淋巴细胞(离体法)测定了三种农药的SCE频率,以了解其遗传效应活性。结果草甘膦无论活体法或离体法均可诱发SCE频率增高,活体法剂量为86mg/kg时,SCE频率为对照组的两倍,表明草甘膦不经代谢活化即有遗传效应活性,经代谢活化后遗传效应增高。敌百虫一尿素液活体法剂量达179mg/kg时,SCE效应为阴性,而离体法可诱发SCE频率增高,表明经代谢后失活。杀虫单活体法可诱发SCE频率增高,但未超过对照组的两倍。
The SCE frequencies of the three pesticides were determined using mouse bone marrow cells (living method) and human lymphocytes (ex vivo) to understand their genetic effect activity. Results The frequency of SCE could be increased in both glyphosate method and in vitro method. When the dose was 86 mg/kg, the SCE frequency was twice that of the control group, indicating that glyphosate had genetic effect without metabolic activation. After the metabolic activation, the genetic effect increased. When the inactivated dose of trichlorfon-urea solution reached 179mg/kg, the SCE effect was negative, while the ex vivo method could induce increased frequency of SCE, indicating that it was inactivated after metabolism. Insecticide single-living method can induce increased SCE frequency, but not more than double the control group.