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目的探讨miR-34b/c rs4938723 T>C与中国人群原发性肝细胞肝癌(hepatocellular carcinoma,HCC)易感性的关系。方法采用病例对照研究设计,选取501例原发性肝细胞肝癌病例和548例正常对照。选取miR-34b/c rs4938723 T>C为研究位点,以聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法进行多态性检测,应用Logistic回归计算OR值及95%CI,比较不同基因型与HCC发病风险的关系。结果在调整年龄、性别、吸烟、饮酒因素后,携带rs4938723 TC/CC基因型者与携带rs4938723 TT基因型个体相比,发生肝癌的风险增加了40%(调整OR=1.40,95%CI=1.10～1.80)。进一步分析显示在原发性肝细胞肝癌的发生中rs4938723基因型与饮酒存在交互作用(相乘交互作用P=0.049,相加交互作用P=0.012)。结论本研究表明位于miR-34b/c启动子区的rs4938723 T>C多态性可能影响中国人群原发性肝细胞肝癌发病风险。
Objective To investigate the relationship between miR-34b / c rs4938723 T> C and susceptibility to primary hepatocellular carcinoma (HCC) in Chinese population. Methods A case-control study was designed and selected 501 cases of primary hepatocellular carcinoma and 548 normal controls. The miR-34b / c rs4938723 T> C was selected as the research site to detect the polymorphism by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Logistic regression was used to calculate the OR and 95% CI , To compare the different genotypes with the risk of HCC. RESULTS: After adjusting for age, sex, smoking, alcohol consumption, the risk of developing HCC was 40% greater in individuals carrying the rs4938723 TC / CC genotype compared with individuals carrying the rs4938723 TT genotype (adjusted OR = 1.40, 95% CI = 1.10 ~ 1.80). Further analysis showed that there was interaction between rs4938723 genotypes and drinking in the occurrence of primary hepatocellular carcinoma (multiplicative interaction P = 0.049, additive interaction P = 0.012). Conclusion This study shows that rs4938723 T> C polymorphism in the miR-34b / c promoter region may affect the risk of primary hepatocellular carcinoma in Chinese population.