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目的:探讨口腔鳞癌患者CD3(+)CD56(+)NKT细胞NKG2D受体抗肿瘤免疫的调节作用。方法:分别从30例口腔鳞癌患者和30例正常人外周血中提取CD3(+)CD56(+)NKT细胞,实时定量PCR和Western印迹分析NKG2D在分子水平和蛋白水平的表达差异。通过siRNA-NKG2D转染方法,分析NKG2D受体对CD3(+)CD56(+)NKT细胞的细胞毒性,分泌IL-2和IFN-μ的影响。采用SPSS13.0软件包对数据进行t检验和方差分析。结果:口腔鳞癌患者CD3(+)CD56(+)NKT细胞NKG2D的表达显著低于正常人(P<0.05);转染siRNA-NKG2D的CD3(+)CD56(+)NKT细胞对肿瘤细胞的杀伤作用显著降低。结论:与正常人相比,口腔鳞癌患者中CD3(+)CD56(+)NKT细胞因NKG2D表达下降,其肿瘤免疫调控作用受到影响。这些发现为NKG2D应用于口腔鳞癌的免疫治疗奠定了基础。
Objective: To investigate the anti-tumor immunity of NKG2D receptor on CD3 (+) CD56 (+) NKT cells in patients with oral squamous cell carcinoma. Methods: CD3 (+) CD56 (+) NKT cells were extracted from peripheral blood of 30 patients with oral squamous cell carcinoma and 30 healthy controls. The expression of NKG2D at the molecular and protein levels was analyzed by real-time PCR and Western blot. The effect of NKG2D receptors on cytotoxicity of CD3 (+) CD56 (+) NKT cells, secretion of IL-2 and IFN-μ was analyzed by siRNA-NKG2D transfection method. SPSS13.0 software package was used for t-test and ANOVA. Results: The expression of NKG2D in CD3 (+) CD56 (+) NKT cells in patients with oral squamous cell carcinoma was significantly lower than that in normal individuals (P <0.05). The CD3 (+) CD56 The killing effect is significantly reduced. Conclusion: The expression of NKG2D on CD3 (+) CD56 (+) NKT cells in oral squamous cell carcinoma patients is lower than that in normal controls, and the tumor immunoregulatory effect is affected. These findings laid the foundation for the immunotherapy of oral squamous cell carcinoma with NKG2D.