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目的对抗-HCV酶免筛查反应性献血者进行确证,制定抗-HCV酶免筛查反应性献血者确证策略。方法2013年12月-2015年9月,收集抗-HCV酶免筛查反应性献血者标本,经ELISA和ID-NAT重新检测,以及追踪和确证检测(RIBA),鉴别献血者筛查抗-HCV检测结果的真、假阳性。结果收集抗-HCV酶免筛查反应性标本73人份,经ELISA和ID-NAT重新检测及追踪和确证后,抗-HCV阳性8份(18.6%),抗-HCV阴性33份(76.7%),抗-HCV不确定2份(4.7%),30份由于未成功追踪而被放弃。抗-HCV筛查反应性献血者抗-HCV真阳性率为10.9%。结论 ELISA和ID-NAT都为反应性时,可以直接判为抗-HCV阳性;单独ELISA反应阳性时,如果RIBA是阳性,判为抗-HCV阳性,如果RIBA是阴性或不确定,3个月后追踪;本研究未发现单独ID-NAT反应性,其追踪方式尚待进一步研究。
Objective To test the anti-HCV enzyme-free screening-positive blood donors and to establish the anti-HCV enzyme-screening-positive response test. Methods From December 2013 to September 2015, anti-HCV enzyme screening-free screening blood samples were collected, re-detected by ELISA and ID-NAT, and follow-up and confirmation test (RIBA) HCV test results are true and false positives. RESULTS: Seventy-three samples of anti-HCV enzyme immunoassay (EMSA) were screened, and 8 anti-HCV positives (18.6%) and 33 anti-HCV negatives (76.7%) were screened by ELISA and ID- ), 2 anti-HCV uncertainties (4.7%) and 30 were dropped due to unsuccessful tracking. The true anti-HCV positive rate of anti-HCV screening reactive blood donors was 10.9%. Conclusions Both ELISA and ID-NAT are anti-HCV positive when they are both reactive. If ELISA alone is positive, they are anti-HCV positive if RIBA is positive or 3 months if RIBA is negative or uncertain Post-tracing; ID-NAT reactivity alone was not found in this study, and its follow-up remains to be further studied.