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目的:建立两种慢性自身免疫性前列腺炎模型,探讨槲皮素(Quercetin)对模型大鼠前列腺组织中炎症因子环氧化酶-2活性(COX-2)、5-脂氧酶(5-LOX)活性、排尿量及尿离子浓度的影响。方法:采用两种自身免疫性前列腺炎大鼠模型:雌二醇诱导的模型(M1)及纯化大鼠前列腺蛋白联合免疫佐剂诱导的模型(M2),分别给药30 d后测定大鼠0~4h排尿量及尿离子浓度(M1);处死大鼠,取前列腺称重计算前列腺指数;取前列腺液镜下观察白细胞数及卵磷脂小体密度;HE染色制备前列腺组织病理切片并镜下观察;Western Blot法及免疫组化法测定前列腺组织内COX-2及5-LOX的表达。结果:两个模型中,槲皮素200 mg/kg给药组前列腺液内卵磷脂小体密度均明显增加,M1中槲皮素200 mg/kg给药组前列腺指数降低,M2中槲皮素200 mg/kg能明显降低前列腺液中的白细胞数;病理切片中均可见病变明显改善;两种模型中前列腺组织内COX-2及5-LOX表达明显被抑制;槲皮素200 mg/kg组对模型大鼠尿液中的Na+和Cl-的排出有明显的促进作用,但对K+的影响并不十分显著(M1);槲皮素(200 mg/kg、100 mg/kg)组能明显增加水负荷大鼠0~4h的排尿量,与对照组比较差别具有统计学意义(M1)。结论:槲皮素可能是通过抑制前列腺组织内COX-2及5-LOX表达从而改善慢性前列腺炎并可能通过促进Na+和Cl-的排出改善模型大鼠排尿困难症状。
Objective: To establish two models of chronic autoimmune prostatitis and investigate the effects of quercetin on the expression of cyclooxygenase-2 (COX-2), 5- LOX) activity, urine output and urinary ion concentration. Methods: Two autoimmune prostatitis rat models: estradiol-induced model (M1) and purified rat prostatic protein combined immunological adjuvant-induced model (M2) were administered 30 d after the determination of rat 0 ~ 4h urinary output and urinary ion concentration (M1); the rats were killed, the prostate was weighed to calculate the prostate index; the number of leukocytes and lecithin density were observed under prostatic fluid microscope; the histological sections of prostate tissue were prepared by HE staining and observed under the microscope The expressions of COX-2 and 5-LOX in prostate tissue were detected by Western Blot and immunohistochemistry. Results: In both models, the density of lecithin in prostatic fluid of quercetin 200 mg / kg group was significantly increased, and the index of prostatic hyperplasia was lower in quercetin 200 mg / kg group in M1, 200 mg / kg can significantly reduce the number of leukocytes in prostatic fluid; pathological changes were seen in lesions significantly improved; two models of prostate tissue COX-2 and 5-LOX expression was significantly inhibited; quercetin 200 mg / kg group The excretion of Na + and Cl- in the urine of rats in model group was significantly promoted (P <0.05), but the effect on K + was not significant (M1); the group of quercetin (200 mg / kg, 100 mg / kg) Urine volume increased from 0 to 4 hours in water-loaded rats, which was statistically significant compared with the control group (M1). CONCLUSIONS: Quercetin may improve chronic prostatitis by inhibiting the expression of COX-2 and 5-LOX in prostate tissue and may improve the symptoms of dysuria in rats by promoting the release of Na + and Cl-.