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目的研究白细胞介素-9(IL-9)单核苷酸多态性与中国汉族人群食管癌患者淋巴结转移易感性的关联。方法采用SNPscan TM高通量基因分型技术,分析355例食管癌患者淋巴结转移组(85例)和无淋巴结转移组(例)IL-9 rs31563 C>T和IL-9 rs31564 G>T这两个多态性位点各基因型分布频率,并计算各基因型的食管癌淋巴结转移风险及其95%可信区间。结果 IL-9 rs31563 C>T基因型分布频率在组间没有统计学差异(P=0.171)。多因素Logistic回归分析,较CC野生纯合型相比,IL-9 rs31563 CT基因型(调整OR=0.58,95%CI=0.30~1.11,P=0.10)和TT基因型(OR及95%CI无数值显示,P=0.99)与食管癌淋巴结转移易感性没有统计学相关性。隐性模型分析结果提示,以CC+CT联合型作为参照,IL-9 rs31563 TT突变纯合型与食管癌淋巴结转移易感性没有统计学关联(OR及95%CI无数值显示,P=0.99)。对IL-9 rs31564 G>T做同样的单基因位点和Logistic回归分析,也未发现其基因型分布频率在淋巴结转移组和无淋巴结转移组之间存在差异,结果也未显示其突变基因型与食管癌淋巴结转移易感性存在统计学意义的关联。结论 IL-9 rs31563 C>T和IL-9 rs31564 G>T基因多态性与食管癌淋巴结转移易感性之间无相关性,还需要进一步的功能学研究及多中心大样本研究对本次实验结果加以验证。
Objective To investigate the association of interleukin-9 (IL-9) single nucleotide polymorphisms with susceptibility to lymph node metastasis in Chinese Han population of esophageal cancer. Methods SNPscan TM high-throughput genotyping was used to analyze the association of IL-9 rs31563 C> T and IL-9 rs31564 G> T in 355 patients with esophageal cancer with lymph node metastasis (85 cases) and without lymph node metastasis The frequency of each genotype was calculated and the risk of lymph node metastasis and its 95% confidence interval for each genotype were calculated. Results There was no significant difference in the distribution frequency of IL-9 rs31563 C> T between the two groups (P = 0.171). Multivariate logistic regression analysis showed that genotypes of IL-9 rs31563 CT (adjusted OR = 0.58, 95% CI = 0.30-1.11, P = 0.10) and TT genotypes (OR and 95% CI No numerical display, P = 0.99) was not statistically associated with susceptibility to lymph node metastasis in esophageal cancer. The results of recessive model analysis showed that there was no statistical relationship between the homozygote of IL-9 rs31563 TT mutation and the susceptibility to lymph node metastasis in esophageal cancer (OR, 95% CI, P = 0.99) with CC + CT combined as reference. . The same single-gene loci and Logistic regression analysis of IL-9 rs31564 G> T did not find any difference in genotype frequency between lymph node metastasis and non-lymph node metastasis, and the results also did not show the mutation genotype There was a statistically significant association with susceptibility to lymph node metastasis in esophageal cancer. Conclusion There is no correlation between IL-9 rs31563 C> T and IL-9 rs31564 G> T gene polymorphisms and susceptibility to lymph node metastasis in esophageal cancer. Further functional studies and large multicenter studies are needed The result is verified.