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目的： 分离伯氏疟原虫敏感株（ Ｃ Ｓ） 与抗氯喹株的氯喹（ｃｈｌｏｒｏｑｕｉｎｅ Ｃ Ｑ） 结合蛋白。方法： 给两株感染鼠ｉｇ Ｃ Ｑ４００ ｍｇ／ｋｇ ， ３ ｈ 后， 收集伯氏疟原虫用（ Ｕｌｔｒｏｇｅｌ Ｒ） Ａｃ Ａ３４ 凝胶柱分离蛋白， 按 Ｂｅｒｇｑｖｉｓｔ 法抽提与蛋白结合的 Ｃ Ｑ， 然后用 Ｈ Ｐ Ｌ Ｃ （ 外标法） 定量。结果： Ｃ Ｓ 有５８ 个蛋白峰， 均与 Ｃ Ｑ 结合， 其中以 Ｎｏ６４ ～８６部分（ 峰９） 结合的 Ｃ Ｑ 量最多， 占总结合量的１６４ ％ 。抗氯喹伯氏疟原虫（ Ｃ Ｒ） 有４０ 个蛋白峰， 除８ 个峰外，均与 Ｃ Ｑ 结合， 结合量最多的蛋白部分与 Ｃ Ｓ相同， 在 Ｎｏ６０ ～８３ 部分（ 峰６ 及峰７） ， 但是它与 Ｃ Ｑ 的结合能力（ Ｃ Ｑｎｍｏｌ／ ｍｇ 蛋白） 显著高于 Ｃ Ｓ。结论： 分离与测定了 Ｃ Ｓ和 Ｃ Ｒ 两株疟原虫各个蛋白峰的分布与定量， 及其与 Ｃ Ｑ 结合的能力。在同一株疟原虫中， 各蛋白与 Ｃ Ｑ 的结合能力存有差异， 在 Ｃ Ｓ与 Ｃ Ｒ 间此差异更大。
OBJECTIVE: To isolate the chloroquine C Q-binding protein of Plasmodium berghei (C S) and chloroquine resistant strains. Methods: Two strains infected with ig C Q400 mg / kg were inoculated for 3 h. The plasmids were isolated from Ultrogel R Ac A34 gel column and the protein-bound C Q was extracted by Bergqvist method. H P L C (external standard method) quantitative. Results: There were 58 protein peaks in C S, all of which were bound to C Q. Among them, No.6 ~ 86 (peak 9) had the highest amount of C Q, accounting for 164% of the total. There were 40 protein peaks in C R, except for 8 peaks, all bound to C Q. The most abundant protein part was the same as that of C S. In No 60 ~ 83 (peak 6 and Peak 7) but its binding capacity to C Q (C Qnmol / mg protein) was significantly higher than that of C S. Conclusion: The distribution and quantitation of each protein peak of C and C R and the ability of binding to C Q were isolated and determined. In the same strain of Plasmodium, there is a difference in the binding capacity of each protein to C Q, and this difference is greater between C S and C R.