烟草上马铃薯Y病毒的提纯与检测

来源 :中国烟草科学 | 被引量 : 0次 | 上传用户:newbitcom
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本文是关于制备马铃薯Y病毒抗血清及ELISA检测技术的实验结果。采用垫层差速离心、蔗糖密度梯度离心提纯PVY,纯化的PVYOD260/OD280为1.56,将提纯的病毒制品免疫家兔,制备抗血清,经微量沉淀及环状沉淀试验,用提纯病毒测定抗血清的效价均为1/2048,并对制备的PVY—IgG作灵敏度及特异性测定。
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