论文部分内容阅读
①目的探讨单纯性肾病综合征（ＳＮＳ）病儿红细胞免疫功能状态及其临床意义。②方法采用红细胞酵母菌花环法和单克隆抗体间接免疫荧光法，检测了２４例ＳＮＳ病儿活动期和缓解期及３０例健康儿童红细胞Ｃ３ｂ受体花环率（Ｅ－Ｃ３ｂＲＲ）和红细胞免疫复合物花环率（Ｅ－ＩＣＲ）及Ｔ细胞亚群的变化。③结果ＳＮＳ病儿活动期和缓解期Ｅ－Ｃ３ｂＲＲ皆显著低于对照组（ｔ＝８．１６，２．４７，Ｐ＜０．０１，０．０５），活动期明显低于缓解期（ｔ＝４．９５，Ｐ＜０．０１），而Ｅ－ＩＣＲ皆无异常；活动期Ｅ－Ｃ３ｂＲＲ降低与ＣＤ＋４细胞和ＣＤ＋４／ＣＤ＋８比值变化呈正相关（ｒ＝０．４８，０．５３，Ｐ＜０．０１），与ＣＤ＋８细胞变化呈负相关（ｒ＝－０．３６，Ｐ＜０．０５），但与激素治疗反应无相关性；缓解期有反复感染、病情反复或复发倾向者，Ｅ－Ｃ３ｂＲＲ较无上述特征者明显降低（ｔ＝２．３８，２．２５，Ｐ＜０．０５）。④结论ＳＮＳ病儿存在原发性红细胞免疫粘附活性降低，Ｅ－Ｃ３ｂＲＲ降低与其缓解期的易感倾向和病情反复或复发密切相关。
Objective To investigate the status of erythrocyte immune function in children with simple nephrotic syndrome (SNS) and its clinical significance. Methods The Erythrocytes yeast rosette method and monoclonal antibody indirect immunofluorescence method were used to detect the E-C3bRR and erythrocyte immune complex of 24 children with SNS during active and remission period and 30 healthy children. Rosette rate (E-ICR) and T cell subsets. Results The E-C3bRR in active and remission stage of SNS children were significantly lower than those in control group (t = 8.16,2.47, P <0.01,0.05), and the activity was significantly lower than that in remission = 4.95, P <0.01), while there was no abnormality in E-ICR. The decrease of E-C3bRR in active stage was positively correlated with the ratio of CD + 4 cells and CD + 4 / CD + 0.01), but negatively correlated with the changes of CD + 8 cells (r = -0.36, P <0.05), but no correlation with hormone therapy response; those with recurrent infection, -C3bRR was significantly lower than those without the above characteristics (t = 2.38,2.25, P <0.05). ④ Conclusion SNS children with primary erythrocyte immune adhesion activity decreased, E-C3bRR decreased with the susceptibility to remission and recurrence or relapse is closely related.