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目的 探讨霍乱毒素 (CTx)对成年金黄地鼠远端视神经受损后视网膜节细胞 (RGCs)再生的作用。方法 远端切断视神经并对接一段自体坐骨神经 (AG) ,玻璃体内注射CTx及 或植入小段坐骨神经分支 (SN)。动物随机分为对照组Ⅰ (AG组 )与对照组Ⅱ (溶剂组 ) ;AG +SN组 ;AG +CTx组 ;AG +SN +CTx组和量效关系组 ,前五组分别存活 4周～ 6周 ,用粒蓝逆行标记再生的RGCs ,荧光显微镜下观察。 结果 AG +CTx组各时间点再生的视网膜节细胞比对照组Ⅰ与对照组Ⅱ明显增加 ,具统计学意义 (P <0 0 5 ) ;AG +SN组得出相似结果。AG +SN +CTx组与其它几组相比在各时间点上均存在极显著性差异 (P <0 0 1)。 结论 霍乱毒素可明显提高远端视神经受损后视网膜节细胞的再生。
Objective To investigate the effect of cholera toxin (CTx) on the regeneration of retinal ganglion cells (RGCs) after the damage of distal optic nerve in adult golden hamster. Methods The optic nerve was severed distally and an autologous sciatic nerve (AG) was intercalated. Intravitreal injection of CTx and / or implantation of a short segment of sciatic nerve branches (SN) were performed. The animals were randomly divided into control group (AG group) and control group Ⅱ (solvent group); AG + SN group; AG + CTx group; AG + SN + CTx group and dose- At 6 weeks, RGCs were retrogradely labeled with granule blue and observed under a fluorescence microscope. Results The retinal ganglion cells regenerated at each time point in AG + CTx group were significantly increased compared with control Ⅰ and control group Ⅱ (P <0.05). Similar results were obtained in AG + SN group. AG + SN + CTx group compared with other groups at all time points there is a significant difference (P <0.01). Conclusion Cholera toxin can significantly improve the regeneration of retinal ganglion cells after distal optic nerve damage.