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目的以博来霉素(Blm)诱导建立硬皮病小鼠模型并加以验证。方法将200μg/mLBlm100μL注射在C3H/He小鼠(C3H小鼠)背部皮下,每日1次,连续3周,建立硬皮病小鼠模型,并经皮肤、肺组织病理学及血清自身抗体检测加以验证。结果注射200μg/mLBlm3周的C3H小鼠,与对照组相比,注射区皮肤厚度明显增加(P<0.001),胶原纤维组织化学指数显著升高(P<0.0001),真皮出现均匀硬化表现;该小鼠肺泡间隔增厚,伴单一核细胞浸润,并可见肺组织小血管壁增厚;血清ANA阳性,间接免疫荧光法检测见细胞核呈粗斑点或细斑点状黄绿色荧光,有的斑点相互聚集成若干大斑点位于核中央,结合抗ENA抗体测定提示存在抗Scl-70抗体、抗着丝点抗体和抗U1-RNP抗体等。结论对C3H小鼠背部每日皮下注射200μg/mLBlm共3周,成功建立了硬皮病小鼠模型。
AIM: To establish and verify a mouse model of scleroderma induced by bleomycin (Blm). Methods A mouse model of scleroderma was established by injecting 100μL of 200μg / mLBlm into the back of the mice in C3H / He mice (C3H mice) once a day for 3 weeks. The skin and lung histopathology and serum autoantibodies To verify. Results C3H mice injected with 200μg / mL Blm3 weeks had markedly increased skin thickness (P <0.001) and collagen fibers (P <0.0001) compared with the control group. The dermis showed uniform sclerosis. Mice with alveolar septum thickening, with mononuclear cell infiltration, and showing thickening of small blood vessels in the lung tissue; serum ANA positive, indirect immunofluorescence test showed the nucleus was coarse spots or fine spot-like yellow-green fluorescence, and some spots gather with each other A number of large spots in the central nucleus, combined with anti-ENA antibody prompted the presence of anti-Scl-70 antibodies, anti-centromere antibodies and anti-U1-RNP antibodies. Conclusion A mouse model of scleroderma was successfully established on the back of the C3H mice by daily subcutaneous injection of 200μg / mLBlm for 3 weeks.