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作者将0.2ml新鲜的同种异体鼠C57BL/6血输入C3H/HEJ鼠体内,然后分别在1、3、7天处死。另一组C3H/HEJ鼠连续3天被输入0.2ml/天同样上述异体鼠血,7天后处死。然后用D10.G4.1 T辅助细胞克隆测定其腹膜巨噬细胞对抗原的表露功能(antigen presentation function)。用白细胞介素(IL-2)依赖的HT-2细胞系测定刀豆素A刺激的脾细胞上清液中IL-2的活性,即脾细胞产生IL-2的功能。从
The authors injected 0.2 ml of fresh allogeneic C57BL / 6 blood into C3H / HEJ mice and sacrificed on days 1, 3 and 7, respectively. Another group of C3H / HEJ mice were injected with 0.2 ml / day of the same rat blood for 3 consecutive days and sacrificed 7 days later. The antigen presentation function of the peritoneal macrophages to the antigen was then determined using the D10.G4.1 T helper cell clone. IL-2 activity in concanavalin A-stimulated spleen cell supernatant was assayed by interleukin (IL-2) dependent HT-2 cell line, ie, the function of spleen cells to produce IL-2. From