论文部分内容阅读
流感嗜血杆菌是急、慢性渗出性中耳炎常见病原菌之一。作者用酶联免疫试验测定非典型流感嗜血杆菌诱发栗鼠患急性渗出性中耳炎过程中血清和中耳渗出物的抗体。动物感染后24小时,所有耳都发展成急性中耳炎,并持续7周。接种活细菌后一周,中耳渗出物抗体的滴度相当于或略高于血清标本的滴度,提示中耳渗出物的抗体是对诱发渗出性中耳炎细菌的反应,可能是在局部产生的。2周后血清和中耳渗出物
Haemophilus influenzae is one of the common pathogens of acute and chronic exudative otitis media. The authors used enzyme-linked immunosorbent assay (ELISA) to determine the antibodies to the serum and middle ear exudates during the acute exudative otitis media induced by atypical Haemophilus influenzae. Twenty-four hours after the animals were infected, all ears developed acute otitis media for 7 weeks. One week after inoculation of viable bacteria, the titer of the middle ear exudate antibody is equivalent to or slightly higher than the titer of the serum sample, suggesting that the antibody of the middle exudate is a response to the bacteria that cause exudative otitis media, possibly at a local produced. Serum and middle ear exudate after 2 weeks