论文部分内容阅读
目的探索近期和慢性弓形虫感染的免疫诊断标志物。方法采用无毒灵敏的TMB为底物的酶联免疫印迹技术,分析弓形虫感染者IgM或IgG抗体阳性血清与弓形虫速殖子可溶性抗原的免疫反应谱。结果IgM抗体对p35、p38和p22的识别比例达80%以上,且反应带出现率较其它抗原组分高,而p32、p30与IgG抗体的反应带出现率和识别比例也分别高达40%和80%以上。其中,反应最强烈最具代表性的分别属p35(出现率81.5%,P<0.0001)和p32(出现率57.1%,P<0.0001)。结论p35、p38、p22可能为近期弓形虫感染的诊断标志物,p32和p30则可能是慢性弓形虫感染的诊断标志物。
Objective To explore immunological diagnostic markers of recent and chronic Toxoplasma infections. Methods Immunosensorial profiles of IgM or IgG antibody-positive sera and Toxoplasma gondii Soluble Toxoplasma gondii Toxoplasma gondii Toxoplasma gondii Toxoplasma gondii Toxoplasma gondii were analyzed by enzyme-linked immunosorbent assay (ELISA) with non-toxic and sensitive TMB as substrate. Results The recognition rate of IgM antibody to p35, p38 and p22 was more than 80%, and the reaction rate was higher than that of other antigen components, while the reaction rate and recognition rate of p32, p30 and IgG antibody were as high as 40% and 80% or more. Among them, the strongest and most representative were p35 (incidence 81.5%, P <0.0001) and p32 (incidence 57.1%, P <0.0001). Conclusion p35, p38 and p22 may be the diagnostic markers of Toxoplasma gondii infection recently, and p32 and p30 may be the diagnostic markers of Toxoplasma gondii infection.