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目的研究螺杆菌感染与原发性肝癌(HCC)的关系。方法选取34例HCC患者的肝癌组织为研究对象(实验组),20例非肝癌手术患者肝组织作对照组。将两组标本制成病理切片,用于螺杆菌属的cDNA-mRNA原位杂交实验。通过定位测定来评价肝组织螺杆菌感染与HCC的相关性。结果利用原位杂交技术检测实验组和对照组标本螺杆菌属16S rRNA-mRNA,实验组有22例阳性,对照组皆为阴性(P<0．01)。进一步用幽门螺杆菌(Hp)和肝螺杆菌(Hh)的cDNA探针进行原位杂交,Hp探针杂交结果与螺杆菌属探针杂交结果相符,阳性率为64．71％,而Hh探针杂交结果为阴性。结论HCC组织中存在Hp感染。
Objective To study the relationship between Helicobacter infection and primary liver cancer (HCC). Methods Totally 34 HCC patients with hepatocellular carcinoma (HCC) were selected as experimental group and 20 HCC patients as control group. Two sets of specimens were made pathological sections for the detection of Helicobacter cDNA-mRNA in situ hybridization. The correlation between H.pylori infection and HCC was evaluated by the localization assay. Results 16S rRNA-mRNA of Helicobacter was detected by in situ hybridization in the experimental group and the control group, 22 cases were positive in the experimental group and negative in the control group (P <0.01). Further in situ hybridization was performed with cDNA probes of Helicobacter pylori (Hp) and Helicobacter pylori (Hh). The hybridization results of Hp probe and Helicobacter probe showed that the positive rate was 64.71% Needle hybridization results were negative. Conclusion There is Hp infection in HCC tissues.