论文部分内容阅读
随着仿生学和生物无机化学的发展,天然酶的模拟工作越来越受到重视,因为模拟酶不仅成本低,而且稳定性好。日本Yutaka Saito等人曾合成MnTPPS_4络合物,替代过氧化物酶作为催化剂用于酚和胺类化合物的氧化显色反应。对Guilbault等人提出的过氧化物酶催化H_2O_2氧化高香草酸的荧光反应,我们曾从底物角度进行研究。本文依据过氧化物酶的活性中心结构,合成了一种金属卟啉化合物,四(4-三甲铵基苯)卟啉和锰的络合物Mn~Ⅲ-T(4-TAP)P,用于模拟辣根过氧化物酶(HRP),催化H_2O_2氧化佳味醇的氧化偶联反应,
With the development of biomimetic and bio-inorganic chemistry, the simulation of natural enzymes is getting more and more attention because the mimic enzymes not only have low cost but also have good stability. Japan Yutaka Saito et al. Have synthesized MnTPPS_4 complex instead of peroxidase as a catalyst for the oxidation and color reaction of phenols and amines. Guilbault et al proposed peroxidase catalyzed H 2 O 2 oxidation of vanillic acid fluorescence reaction, we have studied from the substrate point of view. Based on the active center structure of peroxidase, a complex of metalloporphyrin, tetrakis (4-trimethylaminophenyl) porphyrin and manganese Mn ~ Ⅲ-T (4-TAP) P In the simulated horseradish peroxidase (HRP), the catalytic oxidation of H_2O_2 good taste alcohol oxidation coupling reaction,