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目的检测赖氨酰氧化酶(LOX)在肝癌细胞株中的表达及对肝癌细胞株侵袭性的影响。方法采用实时定量PCR方法检测不同肝癌细胞株中LOX mRNA的表达,Transwell小室侵袭实验观察不同浓度β-氨基丙腈(BAPN)对高侵袭性细胞株SK-Hep-1细胞侵袭性的影响。结果不同细胞株HepG-2、L02、MDA-MB-231、QGY7703、SK-Hep-1、SMMC-7721中LOX mRNA相对表达量依次为3.07、1.00、1.17、0.44、6.49、0.33。LOX mRNA在高侵袭细胞株SK-Hep-1中表达明显高于其他细胞株。加入不同浓度BAPN(0、0.1、0.2、0.3μmol/L,依次为1、2、3、4组)的SK-Hep-1细胞24h后穿膜数分别为(57.33±6.02)、(41.00±3.61)、(19.67±4.04)、(13.33±2.52)个。1组与3组(P<0.05)及与4组(P<0.01)、2组与3组(P<0.05)及与4组(P<0.01)差异均有统计学意义。结论高侵袭性细胞株SK-Hep-1高表达LOX mRNA,降低其LOX活性后侵袭能力明显下降。
Objective To detect the expression of lysyl oxidase (LOX) in hepatocellular carcinoma cell lines and its effect on the invasiveness of hepatocellular carcinoma cell lines. Methods Real-time quantitative PCR was used to detect the expression of LOX mRNA in different hepatocellular carcinoma cell lines. The effect of different concentrations of β-aminopropionitrile (BAPN) on the invasiveness of SK-Hep-1 cells was observed by Transwell chamber invasion assay. Results The relative expression levels of LOX mRNA in HepG-2, L02, MDA-MB-231, QGY7703, SK-Hep-1 and SMMC-7721 cells were 3.07,1.00,1.17,0.44,6.49,0.33. LOX mRNA expression in highly invasive cell line SK-Hep-1 was significantly higher than other cell lines. The number of transmembrane of SK-Hep-1 cells treated with different concentrations of BAPN (0,0.1,0.2,0.3μmol / L, followed by 1,2,3,4) were (57.33 ± 6.02), (41.00 ± 3.61), (19.67 ± 4.04), (13.33 ± 2.52). There was significant difference between group 1 and group 3 (P <0.05) and group 4 (P <0.01), group 2 and group 3 (P <0.05) and group 4 (P <0.01). Conclusions The highly invasive cell line SK-Hep-1 overexpresses LOX mRNA and decreases its LOX activity.