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目的研究PTEN基因在胃癌原发灶及周围淋巴结组织中的表达,以探讨其癌基因在胃癌的发生、发展及转移过程中的作用。方法用免疫组织化学方法检测62例胃癌的原发灶、癌旁组织及周围淋巴结的PTEN表达情况,同时结合病人的病理资料进行分析。结果62例胃癌原发灶PTEN总阳性表达率:41·9%(26/62);癌旁组织为100·0%(62/62);癌转移淋巴结组织中PTEN总阳性表达率:31·4%(27/86);无癌转移淋巴结为92·1%(35/38)。胃癌原发灶PTEN阳性表达率明显低于癌旁组织,而明显高于淋巴结转移癌,差异均有统计学意义(χ2=8·41和χ2=6·75,P<0·01);淋巴结转移癌组织的PTEN阳性表达率低于无癌转移淋巴结,差异有统计学意义(χ2=8·34,P<0·01)。N1、N2站癌转移淋巴结中PTEN的阳性表达率分别为32·1%(18/53)、30·3%(10/33),两者比较差异无统计学意义(χ2=2·31,P>0·05)。原发灶PTEN蛋白阳性表达率与周围淋巴结转移呈负相关。结论抑癌基因PTEN的变化与胃癌的淋巴结转移存在相关关系。
Objective To study the expression of PTEN gene in primary gastric cancer and its peripheral lymph nodes to investigate the role of PTEN in the genesis, development and metastasis of gastric cancer. Methods Immunohistochemistry was used to detect the expression of PTEN in 62 primary gastric cancer tissues, adjacent non-cancerous tissues and surrounding lymph nodes. The pathological data of patients were also analyzed. Results The positive rates of PTEN in 62 cases of primary gastric cancer were 41.9% (26/62) and 100.0% (62/62) respectively. The positive rate of PTEN in the metastatic lymph nodes was 31.0 4% (27/86); no lymph node metastasis was 92.1% (35/38). The positive rate of PTEN in primary gastric cancer was significantly lower than that in paracancerous tissues, but significantly higher than that in lymph node metastases (χ2 = 8.41 and χ2 = 6.75, P <0.01). Lymph nodes The positive rate of PTEN in metastatic carcinoma was lower than that in non-metastatic lymph nodes (χ2 = 8.34, P <0.01). The positive expression rates of PTEN in N1 and N2 metastatic lymph nodes were 32.1% (18/53) and 30.3% (10/33), respectively, with no significant difference (χ2 = 2.31, P> 0.05). The positive rate of PTEN protein in primary tumor was negatively correlated with lymph node metastasis. Conclusion There is a correlation between the change of tumor suppressor gene PTEN and lymph node metastasis in gastric cancer.