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Objective:To isolate and culture PSSCs from different tissues and determine their characteristics anddifferentiation potential in vitro. Methods: PSSCs were isolated and cultured from human aborted fetal bone mar-row, liver, skin, skeletal muscle, lung and pancreas. Morphology and biological activities were assessed. Pheno-types were analyzed by FACS and immunohistochemieal staining. We tested the potential of PSSCs to differentiateinto multiple cell lineages, such as bone, cartilage, fat, muscle, nerve , endothelial cell and hematopoietic progeni-tor cells. Results: PSSCs could be isolated from human aborted fetal above. PSSCs were a population of adherent cells characterized by a typical fibroblast - like morphology. PSSCs had few endoplasmic reticulum and mitochon-drias. It could be expanded by successive cycles of trypsinization, seeding, and culture ex vitro. PSSCs had a capa-bility of passaging up to 30 times without displaying significant changes in morphology, with a 2 -fold increase incell number after each passage. Cell cycle analysis revealed that more than 90 % of cells were in the G0/G1 phases,while a small population of cells were actively engaged in proliferation. These cells were positively stained by FITClabeled CD44, CD29, CD13, but negative for CD34, HLA- DR. The culture- expanded PSSCs have multilineagedifferentiation potential giving rise to cells of osteogenic, chondrogenic, adipogenic, myogenic, neurogenic,hematopoietic and endotielial lineages. Conclusion:PSSCs may still remain in a number of tissues after embryonicdevelopment,could be identified by their phenotypic and functional characteristics, and contribute significantly tomultipotent differentiation outside the tissue of origin.