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利用等离子共振光散射(PRLS)、等离子共振吸收、扫描电子显微镜和动态光散射技术研究了金纳米棒与肝素的相互作用.结果表明,在溶液中,金纳米棒呈分散状态,具有微弱的等离子共振光散射信号.但当其与肝素通过静电作用后发生明显的聚集,产生显著的增强PRLS信号,信号的增强程度与肝素浓度在一定范围内呈线性关系.据此建立了基于金纳米棒聚集测定微量肝素的等离子共振光散射分析法.在60mmol/L NaCl和pH5.33的Britton-Robinson(BR)缓冲溶液介质中,金纳米棒浓度为6.4×10-5mol/L时,测得肝素的线性范围为0.02～0.70μg/mL,检出限为(3σ)8.0ng/mL.该方法成功应用于临床肝素钠注射液的测定.
The interaction between gold nanorods and heparin was studied by using plasma resonance light scattering (PRLS), plasmon resonance absorption, scanning electron microscopy and dynamic light scattering (DLS). The results show that the gold nanorods are dispersed in solution with weak plasma However, when they interact with heparin through electrostatic interaction, a significant increase of PRLS signal is produced, and the enhancement of the signal is in a linear relationship with the concentration of heparin within a certain range.According to this, Determination of trace heparin by plasma resonance light scattering method in 60mmol / L NaCl and pH5.33 Britton-Robinson (BR) buffer solution, the concentration of gold nanorods 6.4 × 10-5mol / L, measured heparin The linear range was 0.02 ~ 0.70μg / mL, the detection limit was (3σ) 8.0ng / mL.The method was successfully applied to the determination of clinical heparin sodium injection.