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单细胞凝胶电泳法（ｓｉｎｇｅｃｅｌｇｅｌｅｌｅｃｔｒｏｐｈｏｒｅｓｉｓ，ＳＣＧＥ）是一种快速，敏感的检测单个哺乳动物细胞ＤＮＡ断裂的技术，也叫彗星实验（ｃｏｍｅｔａｓｓａｙ）．此实验首次通过ＳＣＧＥ法观察抗菌肽ＣＭ４组分对人髓样白血病Ｋ５６２细胞和正常人白细胞核染色质ＤＮＡ的影响，从而进一步研究抗菌肽抗癌作用的机制．荧光显微镜观察显示经抗菌肽ＣＭ４组分处理过的Ｋ５６２癌细胞核染色质ＤＮＡ出现断裂，形成一个亮的荧光头部和彗星似的尾部，而经同样处理的正常人白细胞和未经抗菌肽处理的Ｋ５６２癌细胞核染色质ＤＮＡ未出现断裂，核完整，呈圆形．经彗星尾长分析，前者ＤＮＡ损伤率平均为７３６２％，统计学处理Ｐ＜０００１，具高度显著性差异．这表明，抗菌肽ＣＭ４对Ｋ５６２癌细胞核染色质ＤＮＡ有明显的断裂作用，而对正常人白细胞则没有断裂作用．
Single-cell gel electrophoresis (SCGE) is a rapid and sensitive technique for detecting DNA fragmentation in a single mammalian cell, also known as cometassay. This experiment for the first time by SCGE observation CM4 components of human myeloid leukemia K562 cells and normal human leukocyte nuclear chromatin DNA in order to further study the anti-cancer mechanism of antimicrobial peptides. Fluorescence microscopy showed that the nuclear chromatin DNA of K562 cancer cells treated with the antimicrobial peptide CM4 fragment broke to form a bright fluorescent and cometlike tail, whereas normal white blood cells treated with the same treatment were not treated with antimicrobial peptides K562 cancer cell nuclear chromatin DNA did not appear broken, nuclear integrity, was round. The tail length of the comet analysis, the former DNA damage rate was 73 62% on average, the statistical treatment P <0 001, with a highly significant difference. This indicates that the antibacterial peptide CM4 has obvious cleavage effect on K562 cancer cell nuclear chromatin DNA and no cleavage effect on normal human leukocytes.