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目的 建立解脲脲原体（Ｕｕ）小鼠宫内感染的动物模型。方法 分别将４、１ 和９ 型Ｕｕ（２×１０４ ｃｆｕ／ｍ ｌ）接种于妊娠第１２．５、１３．５、１４．５ 天孕鼠阴道内（３４ 只），以最后一次攻击后的４８ｈ 内分娩为早产。以腹腔内接种４型Ｕｕ（６ 只）和阴道内注入灭菌盐水（９ 只）为对照组。结果 经阴道内接种Ｕｕ４型的４３．７％ （７／１６）孕鼠在４８ｈ 内早产，显著高于１ 型Ｕｕ（８．３％ ，１／１２，Ｐ＜ ０．０５）和９型Ｕｕ（０％ ，０／６，Ｐ＜ ０．０５）以及对照组（０％ ，０／１５，Ｐ＜ ０．０１）；接种４、１ 和９型Ｕｕ 后，早产和不早产孕鼠子宫角Ｕｕ 的检出率分别为９７％ 、４６％ 和２５％ ；接种４ 型Ｕｕ 后，早产幼鼠的心、肺Ｕｕ 检出率为４６％ （２５／５４），而注射灭菌盐水、１和９ 型Ｕｕ 的非早产幼鼠中Ｕｕ 的检出率均为０％ （０／４５，０／５５，０／４５，Ｐ＜ ０．０１）。三种血清型Ｕｕ 感染后早产或不早产幼鼠脑组织中Ｕｕ 的检测均为０％ （０／５９）。结论 小鼠Ｕｕ４ 型和１型阴道内接种可导致部分孕鼠宫内感染动物模型的建立将有助于对人类Ｕｕ 早产发病机制的进一步研究。
Objective To establish an animal model of intrauterine infection of Uu mice. Methods Uu (2 × 104 cfu / ml) of type 4, 1 and 9 were inoculated intravaginally (34) on the 12th, 13th, 14th and 14th day of pregnancy respectively. After the last challenge 48h delivery for premature delivery. Intraperitoneal inoculation type 4 Uu (6) and intravaginal injection of sterile saline (9) as the control group. Results 43.7% (7/16) of pregnant rats injected intravaginally with Uu4 were prematurely born within 48 hours, which were significantly higher than those of Uu type 1 (8.3%, 1/12, P <0.05) and type 9 Uu (0%, 0/6, P <0.05) and control group (0%, 0/15, P <0.01) The detection rate of Uu was 97%, 46% and 25%, respectively. After Uu 4 was inoculated, the detection rate of heart and lung Uu in premature delivery was 46% (25/54) The detection rate of Uu in type 9 Uu non-preterm young rats was 0% (0/45, 0/55, 0/45, P <0.01). Uu was detected in 0% (0/59) brain tissue of preterm or nonpremature infants after infection with three serotypes of Uu. Conclusion Intrauterine inoculation of mice with type Uu4 and type 1 can lead to the establishment of animal models of intrauterine infection in some pregnant rats and will be helpful for the further study on the pathogenesis of human uu preterm birth.