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目的 探讨乙型肝炎病毒载量与抗原抗体模式的关系,为乙型肝炎的诊治、预防提供科学依据。方法用实时荧光定量聚合酶链反应和ELISA检测HBV DNA和抗原抗体,统计分析不同抗原抗体模式的HBV DNA量。结果 在HBsAg和HBeAg阳性模式中,HBV DNA拷贝数>103/ml者占87.3％,其中>107/ml者占66.7％;在HBsAg阳性,抗-HBe阳性模式中,HBV DNA拷贝数<103/ml者占74.5％,>107/ml者占8.3％;在HBsAg阳性,抗-HBc阳性模式中,HBV DNA拷贝数>103/ml者占48.0％,>107/ml者占29.1％。HBeAg阳性模式HBV载量显著高于HBeAg阴性模式（P<0.01）。抗-HBe阴性模式 HBV载量显著高于抗-HBe阳性模式（P<0.01）。在HBsAg阴性模式中,HBV DNA拷贝数>103/ml占 7.9％,1例拷贝数高达1.59× 109/ml。结论HBeAe阳性模式HBV载量显著高于HBeAe阴性模式,抗-HBe阴性模式病毒载量显著高于抗-HBe阳性模式,HBeAg阳性模式中的少数人HBV载量很低,HBeAg阴性模式中少数人HBV载量很高,HBsAg阴性模式中少数人存在病毒复制,因此,对于一具体患者来说,根据抗原抗体模式,难以准确地判断病毒复制程度及其传染性的强弱。
Objective To investigate the relationship between hepatitis B virus load and antigen-antibody pattern and to provide a scientific basis for the diagnosis and treatment of hepatitis B. Methods HBV DNA and antigen antibodies were detected by real-time fluorescence quantitative polymerase chain reaction and ELISA. The amounts of HBV DNA in different antigen-antibody patterns were statistically analyzed. Results HBV DNA copy number> 103 / ml accounted for 87.3%, of which> 107 / ml accounted for 66.7% in HBsAg and HBeAg positive mode; HBV DNA copy number <103 / ml, accounted for 74.5%, and those with> 107 / ml accounted for 8.3%. HBV DNA copy number> 103 / ml accounted for 48.0% and> 107 / ml accounted for 29.1% in HBsAg positive and anti-HBc positive models. The HBeAg-positive HBV load was significantly higher than that of HBeAg-negative (P <0.01). Anti-HBe negative HBV load was significantly higher than anti-HBe positive (P <0.01). In the HBsAg-negative model, HBV DNA copy number> 103 / ml accounted for 7.9%, 1 copy number as high as 1.59 × 109 / ml. Conclusions The HBeAe positive model HBV load is significantly higher than that of HBeAe negative model, the anti-HBe negative model viral load is significantly higher than the anti-HBe positive mode, a small number of HBeAg-positive mode of HBV load is very low, a small number of HBeAg negative mode The HBV load is high, and a small number of people in the HBsAg negative mode have viral replication. Therefore, according to the antigen-antibody model, it is difficult to accurately determine the degree of virus replication and the extent of its infectivity for a specific patient.