论文部分内容阅读
布鲁杆菌病的病原体是布鲁氏杆菌,一种胞内寄生菌。该病是典型的人畜共患病。布鲁氏菌病全球多发,对布鲁氏菌易感动物源头的防控,可以有效的降低人群的患病率~([1])。如果要建立PCR分子诊断方法,需要对该方法做出敏感性、特异性、高效性等评估。本实验对布鲁氏菌PCR检测方法、血清学平板凝集检测方法和病菌分离培养方法做出比较评估。1资料与方法1.1样品采集样品主要从疑似患病人群中随机采
The brucellosis pathogen is Brucella, an intracellular parasite. The disease is a typical zoonosis. Multiple brucellosis worldwide, the prevention and control of brucellosis-prone animal origin, can effectively reduce the prevalence of the crowd ~ ([1]). If you want to establish a molecular diagnostic method of PCR, the method needs to be evaluated for its sensitivity, specificity and efficiency. In this experiment, Brucella PCR detection methods, serological plate agglutination detection methods and pathogenic bacteria isolation and culture methods to make a comparative assessment. 1 Materials and Methods 1.1 Sample Collection Samples from the suspected population of patients randomly collected