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目的:观察热休克蛋白70(HSP70)在心肌缺血再灌注损伤过程中的表达规律及银杏叶提取物(EGB)干预后的变化。方法:65只SD大鼠随机分为假手术组、模型组和治疗组。试验前30 min,治疗组大鼠腹腔注射银杏叶提取物50 mg/kg,结扎左冠状动脉前降支建立大鼠急性心肌缺血再灌注损伤模型。于再灌注不同时间点(1、3、6、12、24、48 h)取出心脏左前降支支配区的全层心肌组织,用免疫组织化学和Western Blot方法观察心肌HSP70的表达。结果:心肌缺血再灌注损伤时HSP70主要表达在心肌细胞和微血管上,表达水平随再灌注时间延长而增加,24 h时达高峰;与模型组相比,治疗组心肌组织HSP70表达水平明显上调,表达时间明显延长。结论:EGB促进HSP70在心肌表达增强和延长,可能与其提高心肌对缺血再灌注损伤的耐受性有关。
OBJECTIVE: To observe the expression of heat shock protein 70 (HSP70) during myocardial ischemia-reperfusion injury and the changes after intervention of Ginkgo biloba extract (EGB). Methods: Sixty-five SD rats were randomly divided into sham operation group, model group and treatment group. 30 min before the experiment, the rats of the treatment group were injected with Ginkgo biloba extract 50 mg/kg by intraperitoneal injection and the left anterior descending coronary artery was ligated to establish a rat model of acute myocardial ischemia-reperfusion injury. At the different time points of reperfusion (1, 3, 6, 12, 24, 48 h), the full-thickness myocardial tissue in the left anterior descending branch of the heart was removed and the expression of HSP70 in the myocardium was observed by immunohistochemistry and Western Blot. RESULTS: Myocardial ischemia/reperfusion injury induced the expression of HSP70 mainly on cardiomyocytes and microvessels. The expression level increased with the increase of reperfusion time and peaked at 24 hours. Compared with the model group, the expression of HSP70 in myocardium of the treated group was significantly increased. , The expression time is obviously extended. CONCLUSION: EGB promotes the expression and prolongation of HSP70 in myocardium, which may be related to the enhancement of myocardial tolerance to ischemia-reperfusion injury.