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目的研究乳酸菌抗氧化活性与缓解葡聚糖硫酸钠(DSS)诱导结肠炎的关系。方法评价5株乳酸菌体外抑制大鼠肝细胞脂质过氧化活性,用DSS诱导小鼠结肠炎模型研究抗氧化性不同的菌株体内抗氧化活性;小鼠分成7组,分别是Control组、DSS组、L.plantarum Fn008+DSS组、L.casei Fn012+DSS组、L.acidophilus Fn022+DSS组、L.sakei Fn034+DSS组和L.acidophilus Fn037+DSS组。结果体外实验表明,Fn022和Fn034的丙二醛(MDA)抑制率高于LGG,但差异无统计学意义,Fn008和Fn037的MDA抑制率显著低于LGG,Fn012的MDA抑制率最低。体内实验表明,DSS可导致小鼠氧化应激,升高结肠内容物的自由基水平,降低结肠抗氧化能力;Fn008和Fn037预处理能显著降低DSS诱导结肠内容物的自由基水平,Fn008能显著降低结肠组织的髓过氧化物酶(MPO)水平,提高结肠抗氧化能力,Fn022和Fn034的干预能力居中,Fn012的干预能力最弱。相关性分析表明乳酸菌体外MDA清除率与体内MPO有负相关趋势(r=-0.862,P=0.06)。结论乳酸菌抗氧化活性与其在肠道抑制炎性细胞激活的作用有关,本实验中Fn008和Fn037具有显著的体内抗氧化作用。
Objective To study the relationship between antioxidant activity of lactic acid bacteria and relieving colitis induced by dextran sodium sulfate (DSS). Methods Five strains of lactic acid bacteria were used to evaluate the activity of lipid peroxidation in rat hepatocytes in vitro. The anti-oxidative activity of different strains of anti-oxidative strains was studied by DSS-induced mouse colitis model. The mice were divided into 7 groups: Control group, DSS group L. plantarum Fn008 + DSS group, L.casei Fn012 + DSS group, L. acidophilus Fn022 + DSS group, L.sakei Fn034 + DSS group and L. acidophilus Fn037 + DSS group. Results In vitro experiments showed that the inhibitory rate of malondialdehyde (MDA) of Fn022 and Fn034 was higher than that of LGG, but the difference was not statistically significant. The inhibitory rate of MDA of Fn008 and Fn037 was significantly lower than that of LGG, and the inhibition rate of Fn012 was the lowest. In vivo experiments show that DSS can lead to oxidative stress in mice, increasing free radicals in colon contents and decreasing colon anti-oxidative capacity. Pretreatment with Fn008 and Fn037 can significantly reduce free radical levels in colon induced by DSS, and Fn008 can significantly Reduce the level of myeloperoxidase (MPO) in colonic tissue and increase the anti-oxidative capacity of colonic mucosa. The intervening ability of Fn022 and Fn034 was the center, and the intervening ability of Fn012 was the weakest. Correlation analysis showed that there was a negative correlation between lactobacilli MDA clearance rate and in vivo MPO (r = -0.862, P = 0.06). Conclusion The antioxidant activity of lactic acid bacteria is related to its role in intestinal inflammatory cell activation. In this experiment, Fn008 and Fn037 have significant anti-oxidative activity in vivo.