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目的探讨白细胞介素1β(IL-1β)-5l1位点C/T多态性与慢性心力衰竭(CHF)发病关系。方法CHF组及健康对照组,每组80例,抽取静脉血5 mL,提取基因组DNA,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,分析IL-1β-511位点基因多态性。结果等位基因T在CHF组检出率为56.25%,明显高于健康对照组检出率的38.75%,差异有统计学意义(P<0.05)。结论 IL-1β-511 C/T多态性与CHF发病具有相关性,等位基因T可能是部分CHF患者易感性遗传标志。
Objective To investigate the relationship between interleukin-1β (IL-1β) -5l1 C / T polymorphism and the incidence of chronic heart failure (CHF). Methods CHF group and healthy control group, 80 cases in each group, venous blood was drawn from 5 mL, and genomic DNA was extracted. PCR-RFLP was used to detect IL-1β-511 Point gene polymorphism. Results The detection rate of allele T in CHF group was 56.25%, which was significantly higher than that in healthy control group (38.75%), the difference was statistically significant (P <0.05). Conclusion The IL-1β-511 C / T polymorphism is associated with the pathogenesis of CHF. The allele T may be a genetic marker of susceptibility in some CHF patients.