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目的:探索血小板糖蛋白(glycoprotein,Gp)ⅡbⅢa复合体的基因多态性是否与动脉硬化性脑血栓形成有关。方法:GpⅡb组病例122例,对照组99例,GpⅢa组病例127例,对照组104例,提取空腹静脉血白细胞中DNA,经聚合酶链反应(PCR)后,分别用限制性内切酶FokⅠ、ScrFⅠ酶切,2.2%琼脂糖凝胶电泳,紫外分析仪观察。数据统计应用SPSS11.5forWindows统计软件包。结果:GpⅡb组中≤70岁的人GpⅡbSer843等位基因的纯合子型与增加动脉硬化性脑血栓形成的危险相关(P=0.029);将病例组和对照组中的男性,按照基因型bb组和(aa+ab)组进行分析,结果显示两组间有显著差异(P=0.01,OR=2.194,95%CI=1.177～4.091);GpⅢa组共231例均为GpⅢaPlA1基因型。结论:在动脉硬化性脑血栓形成中有GpⅡbIle/Ser843的基因多态性异常;而GpⅢaPlA1基因型无异常。
Objective: To explore whether the gene polymorphism of glycoprotein Ⅱb Ⅲ a complex is associated with arteriosclerotic cerebral thrombosis. Methods: 122 cases of GpⅡb group, 99 cases of control group, 127 cases of GpⅢa group and 104 cases of control group were collected. The DNA of fasting venous blood leucocytes was extracted by polymerase chain reaction (PCR) , ScrF Ⅰ digestion, 2.2% agarose gel electrophoresis, UV analyzer observation. Data Statistics Application SPSS11.5forWindows statistical package. Results: The homozygote of GpⅡbSer843 allele in GpⅡb group ≤70 years old was associated with an increased risk of atherothrombotic cerebral thrombosis (P = 0.029). The males in case group and control group were divided according to genotype bb (P = 0.01, OR = 2.194, 95% CI = 1.177-4.091). A total of 231 cases in the GpIIIa group were all GpIIIaPlA1 genotypes. Conclusion: GpⅡbIle / Ser843 gene polymorphism is abnormal in arteriosclerotic cerebral thrombosis, while GpⅢaPlA1 genotype is not abnormal.