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目的：了解紫杉醇对喉鳞状细胞癌细胞株Ｈｅｐ－２生长抑制作用，为治疗喉癌提供实验依据。方法：应用肿瘤细胞培养技术，随机分组和空白对照设计，观察紫杉醇对喉癌细胞生长抑制的剂量和时间效应。培养４８ｈ后作流式细胞仪分析，观察细胞周期分布和凋亡发生率。结果：紫杉醇浓度为１×１０－８组的癌细胞全部死亡，２．５×１０－９的低浓度已能明显影响细胞的生长，５×１０－９浓度组的细胞生长受到明显抑制。细胞周期分析显示：在中等或低浓度紫杉醇作用下，Ｈｅｐ－２细胞被阻止于Ｇ０／Ｇ１期，并能诱导细胞发生凋亡。结论：喉癌细胞株Ｈｅｐ－２对紫杉醇是高度的敏感，能使细胞生长阻止于Ｇ０／Ｇ１期，并诱导细胞凋亡，为紫杉醇治疗喉癌提供可靠的实验依据。
OBJECTIVE: To investigate the inhibitory effect of paclitaxel on the growth of laryngeal squamous cell carcinoma cell line Hep-2 and provide experimental evidence for the treatment of laryngeal cancer. Methods: Tumor cell culture technique, randomized control group and blank control design were used to observe the dose and time effect of paclitaxel on the growth inhibition of laryngeal carcinoma cells. 48h after culture for flow cytometry analysis of cell cycle distribution and the incidence of apoptosis. Results: All the cancer cells in the group of 1 × 10-8 paclitaxel died. The low concentration of 2.5 × 10-9 could obviously affect the growth of the cells, and the cell growth in 5 × 10-9 group was significantly inhibited. Cell cycle analysis showed that Hep-2 cells were blocked in the G0 / G1 phase under the moderate or low concentration of paclitaxel and could induce cell apoptosis. Conclusion: Hep-2 laryngeal carcinoma cell line is highly sensitive to paclitaxel, which can prevent cell growth in G0 / G1 phase and induce apoptosis, providing a reliable experimental basis for paclitaxel treatment of laryngeal cancer.