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ObjectiveTo investigate the changes in chemical constituents of Radix Bupleuri before and after processing. Methods The chemical constituents of Radix Bupleuri were detected before and after processing with UV,HPLC and TLC methods. ResultsThe chromatographic conditions were determined as follows: chromatographic column of Kromasil C 18 ( 4.6 mm ×250 mm,5 μm) ,column temperature of 25 ℃ ,mobile phase of acetonitrile ( A) water ( B) ,gradient dilution of phase A at 10% within 0 3 min,phase A at 10% 15% within 3 5 min,phase A at 15% 25% within 5 20 min,phase A at 25% 40% within 20 30 min,phase A at 40% 55% within 30 40 min,phase A at 55% 75% within 40 45 min,phase A 75% 90% within 45 60 min. Under these parameters,the chemical constituents could be well separated. Comparison of HPLC fingerprints showed that chemical constituents in raw and processed Radix Bupleuri were changed remarkably,and some new constituents produced after processing. Thin layer chromatography conditions were determined as follows: expansion system of chloroform methanol water ( 100∶20∶1,V/V/V) ,chromogenic agent of 5% vanillin-sulfuric acid to heat to 105 ℃ till the appearance of clear spots. The spot location of TLC fingerprints of chemical constituents differed between raw and processed Radix Bupleuri. ConclusionUV,HPLC and TLC all confirmed the changes of chemical constituents of Radix Bupleuri before and after processing.
ObjectiveTo investigate the changes in chemical constituents of Radix Bupleuri before and after processing. Methods The chemical constituents of Radix Bupleuri were detected before and after processing with UV, HPLC and TLC methods. Results The chromatographic conditions were determined as follows: chromatographic column of Kromasil C 18 (4.6 mm × 250 mm, 5 μm), mobile phase of acetonitrile (A) water (B), gradient dilution of phase A at 10% within 0 3 min, phase A at 10% 15% within Phase A at 15% 25% within 5 20 min, phase A at 25% 40% within 20 30 min, phase A at 40% 55% within 30 40 min, phase A at 55% 75% within 40 45 min, phase A 75% 90% within 45 60 min. Comparison of HPLC fingerprints showed that chemical constituents in raw and processed Radix Bupleuri were changed remarkably, and some new constituents produced after processing . Thin layer chromatography conditions were det ermined as follows: expansion system of chloroform methanol water (100: 20: 1, V / V / V), chromogenic agent of 5% vanillin-sulfuric acid to heat to 105 ° C till the appearance of clear spots. fingerprints of chemical constituents differed between raw and processed Radix Bupleuri. Conclusion UV, HPLC and TLC all confirmed the changes of chemical constituents of Radix Bupleuri before and after processing.