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AIM:To detect the effects of plasma DNA methylation of Wnt antagonists/inhibitors on recurrence of esophageal squamous cell carcinoma(ESCC).METHODS:We used methylation-specific polymerase chain reaction to detect hypermethylation of the promoter of four Wnt antagonists/inhibitors(SFRP-1,WIF-1,DKK-3 and RUNX3) using DNA from the plasma of ESCC patients(n = 81) and analyzed the association between promoter hypermethylation of Wnt pathway modulator genes and the two-year recurrence of ESCC.RESULTS:Hypermethylation of SFRP-1,DKK-3 and RUNX-3 was significantly associated with an increased risk of ESCC recurrence(P = 0.001,0.003 and 0.001 for SFRP-1,DKK-3 and RUNX3,respectively).Patients carrying two to three methylated genes had a significantly elevated risk of recurrence compared with those not carrying methylated genes(odds ratio = 15.69,95% confidential interval:2.97-83).The area under the receiver operating characteristic curve(AUC) was 77.1 for ESCC recurrence prediction(sensitivity = 66.67 and specificity = 83.3).When combining methylated genes and the clinical stage,the AUC was 83.69,with a sensitivity of 76.19 and a specificity of 83.3.CONCLUSION:The status of promoter hypermethylation of Wnt antagonists/inhibitors in plasma may serve as a non-invasive prognostic biomarker for ESCC.
AIM: To detect the effects of plasma DNA methylation of Wnt antagonists / inhibitors on recurrence of esophageal squamous cell carcinoma (ESCC). METHODS: We used methylation-specific polymerase chain reaction to detect hypermethylation of the promoter of four Wnt antagonists / inhibitors -1, WIF-1, DKK-3 and RUNX3) using DNA from the plasma of ESCC patients (n = 81) and analyzed the association between promoter hypermethylation of Wnt pathway modulator genes and the two-year recurrence of ESCC.RESULTS: Hypermethylation of SFRP-1, DKK-3 and RUNX-3 were significantly associated with an increased risk of ESCC recurrence (P = 0.001, 0.003 and 0.001 for SFRP- 1, DKK- 3 and RUNX3, respectively). Patients carrying two to three methylated genes had a significantly elevated risk of recurrence compared with those not carrying methylated genes (odds ratio = 15.69, 95% confidential interval: 2.97-83). The area under the receiver operating characteristic curve (AUC) was 77.1 for ESCC recurrence prediction = 66.67 and specificity = 83.3) .When combining methylated genes and the clinical stage, the AUC was 83.69, with a sensitivity of 76.19 and a specificity of 83.3. CONCLUSION: The status of promoter hypermethylation of Wnt antagonists / inhibitors in plasma may serve as a non-invasive prognostic biomarker for ESCC.