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探讨乙型肝炎病毒(HBV)基因组异质性及其生物学意义。以已知HBV基因序列为依据 ,设计特异性多聚酶链反应 (PCR)引物 ,自 2例慢性HBV感染患者外周血血清中扩增HBV基因组序列 ,克隆入pGEMTeasy质粒 ,随机挑选克隆进行DNA测序并加以比较。测序结果发现 ,来源不同的 5株HBV全基因组核苷酸序列的一致率为 93 6 % ,不同克隆的 4个开放读码框架长度有明显区别 ,氨基酸序列中存有移框、缺失、替换等多种变异类型。说明来源于同一患者的HBV基因组序列之间存在有较明显的变异现象 ,在患者体内构成了准种群。
To investigate the genomic heterogeneity of Hepatitis B virus (HBV) and its biological significance. Based on the known HBV gene sequences, specific polymerase chain reaction (PCR) primers were designed and HBV genome sequences were amplified from the peripheral blood serum of 2 patients with chronic HBV infection. The PCR products were cloned into pGEMTeasy plasmid and cloned randomly for DNA sequencing. Compare Sequencing results showed that the identities of the nucleotide sequences of the five HBV genomes from different origins were 93.6%, and the lengths of the four open reading frames of different clones were significantly different. There were frame-shifting, deletion, substitution, etc. in the amino acid sequence Variety of mutation types. This shows that there is a more obvious variation among the HBV genomic sequences from the same patient and constitutes a quasi-population in the patient.