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目的研究较长时间吸入甲醛小鼠记忆力的改变,并初步阐明其作用机制。方法采用健康昆明种小鼠,按照随机分组的原则,将小鼠分为对照组和甲醛染毒组,染毒组浓度分别是21.0mg/m3(1/24LC50)、42.0mg/m3(1/12LC50)和84.0mg/m3(1/6LC50),每组12只,雌雄各半。采用静式吸入染毒,每天2h,每周6d,染毒12周,对照组吸入正常空气。染毒第6、8、12周d1,进行小鼠记忆力的测试。染毒结束后处死小鼠,检测脑组织SOD、GSH-Px及MDA含量。结果在记忆力测试中,第8周,84.0mg/m3组小鼠游泳到达目的地时间大于对照组(P﹤0.05);第12周,42.0mg/m3组和84.0mg/m3组小鼠游泳到达目的地时间大于对照组(P﹤0.01),84.0mg/m3组小鼠游泳到达目地时间也大于21.0mg/m3组(P﹤0.01),84.0mg/m3组小鼠较第8周游泳到达目的地时间延长(P﹤0.01)。甲醛对小鼠脑组织脂质过氧化作用也有影响,84.0mg/m3组小鼠SOD活性低于对照组和21.0mg/m3组(P﹤0.05);各染毒组GSH-Px活性低于对照组(P﹤0.05或P﹤0.01);各染毒组MDA含量与对照组比较均增加(P﹤0.01)。结论小鼠较长时间接触甲醛可引起记忆力的降低,脑组织抗氧化物酶SOD和GSH-Px活性下降,脂质过氧化产物MDA含量增加,提示脂质过氧化损伤可能是小鼠记忆力降低的机制之一。
Objective To study the changes of memory in mice inhaled for a long time and clarify its mechanism. Methods Healthy Kunming mice were randomly divided into control group and formaldehyde exposure group according to the principle of randomization. The concentrations of exposure groups were 21.0mg / m3 (1 / 24LC50) and 42.0mg / m3 (1 / 12LC50) and 84.0mg / m3 (1/6 LC50), each group of 12, male and female. The rats were exposed to static inhalation for 2 hours a day, 6 days a week for 12 weeks. The control group inhaled normal air. The 6th, 8th and 12th week d1 were tested for memory of mice. The mice were sacrificed after the end of the exposure and the contents of SOD, GSH-Px and MDA in the brain tissue were measured. Results At the 8th week, the swimming time of 84.0mg / m3 swimming group was higher than that of the control group (P <0.05). At the 12th week, the mice swimming in 42.0mg / m3 group and 84.0mg / m3 group arrived The target time was longer than that of the control group (P <0.01). The swimming time of the 84.0 mg / m3 group reached more than 21.0 mg / m3 (P <0.01). The 84.0 mg / Time extended (P <0.01). Formalin also had an effect on lipid peroxidation in mice brain. SOD activity in 84.0mg / m3 group was lower than that in control group and 21.0mg / m3 group (P <0.05). GSH-Px activity in each group was lower than that in control group Group (P <0.05 or P <0.01). The content of MDA in each exposure group increased compared with the control group (P <0.01). Conclusions Mice exposed to formaldehyde for a long time can cause the decrease of memory, the activity of antioxidant enzymes SOD and GSH-Px in brain tissue and the increase of MDA content in lipid peroxidation products, suggesting that the lipid peroxidation injury may be the decrease of memory in mice One of the mechanisms.