基因型和供体植株生长环境对大白菜游离小孢子胚胎发生的影响

• 作 者: 曹鸣庆 李岩 刘凡
• 机 构: 北京市农林科学院蔬菜研究中心,北京市农林科学院蔬菜研究中心,北京市农林科学院蔬菜研究中心 北京100081,北京100081,北京100081
• 出 处: 华北农学报
• 发表日期: 1993年04期
• 关键词: 小孢子 游离小孢子培养 胚胎发生 生长温度 单倍体植株 液体培养基 花药培养 温度处理 频率方面 心形胚

17个用于大白菜常规育种的基因型在NLN-13液体培养基中进行游离小孢子培养,结果有16个基因型经由小孢子胚胎发生途径获得小孢子胚.这些基因型包括从早熟到晚熟的各种类型.各基因型之间在小孢子胚胎发生频率方面存在很大差异,产量最高的两个基因型T11和CC11,平均每百花蕾分别达35 928和34 132个胚.供体植株生长环境对小孢子胚胎发生具有明显影响:在10/20℃(夜/昼)的部分控温温室生长的供体植株,其小孢子胚产量较高;而在温度无法调控、午间最高温达30-40℃、夜间降至10-15℃左右田间生长的供体植株,胚产量仅为前者的29.5%.

Sixteen genotypes of microspore embryos were obtained by microspore embryogenesis from 17 microspores cultured in NLN-13 broth, the genotypes of which were used for routine breeding in Chinese cabbage.These genotypes included from early maturity to late maturity The genotypes differed greatly in the frequency of microspore embryogenesis, with the highest yielding genotypes T11 and CC11 with an average of 35,928 and 34,132 embryos per hundred flower buds, respectively The growth environment had a significant effect on microspore embryogenesis. The donor plants grown in partial temperature-controlled greenhouse at 10/20 ℃ (night / day) had higher microsporum embryo yield, but at temperatures not controllable, 30-40 ℃, night to 10-15 ℃ around the donor plants grown in the field, embryo production was only 29.5% of the former.