高效液相色谱法测定补脾益肠胶囊中补骨脂素和异补骨脂素的含量

• 作 者: 王婧璨 柯学 刘丽芳
• 机 构: 中国药科大学中药分析教研室,中国药科大学药剂教研室,
• 出 处: 中国实用医药
• 发表日期: 2009年01期
• 关键词: 补脾益肠胶囊 补骨脂素 异补骨脂素 高效液相色谱法

目的建立补脾益肠胶囊中补骨脂素和异补骨脂素的含量测定方法。方法Hedera ODS C18(250mm×4.6mm,5μm)色谱柱,流动相为甲醇∶水(58∶42),检测波长为245nm。结果补骨脂素的线性范围为0.020～0.30μg,r=0.9998(n=7),平均加样回收率为100.61%,RSD为1.86%(n=5);异补骨脂素的线性范围为0.038～0.266μg,r=0.9999(n=7),平均加样回收率为100.76%,RSD为1.60%(n=5)。结论该方法简便,准确,重现性好,可以应用于补脾益肠胶囊的质量控制。

Objective To establish a method for the determination of psoralen and isopsoralen in Bupisyanyi Capsule. Method Hedera ODS C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was methanol: water (58:42) and the detection wavelength was 245 nm. Results The linear range of psoralen was 0.020-0.30μg, r = 0.9998 (n = 7), the average recovery was 100.61% and the RSD was 1.86% (n = 5). The linear range of isopsoralen Was 0.038-0.226μg, r = 0.9999 (n = 7). The average recovery was 100.76% and the RSD was 1.60% (n = 5). Conclusion The method is simple, accurate and reproducible. It can be used for quality control of Bupi Yikou capsule.