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目的筛选一种可准确、高效鉴别千斤拔属Flemingia Roxb.ex Ait.et Ait.f.药用植物的DNA条形码序列。方法对4种14份千斤拔属药用植物的ITS2、rbc L、psb A-trn H序列进行扩增和测序,同时查找NCBI数据库中千斤拔属植物的相应序列,共计7种20份,比较不同序列的扩增效率及测序成功率,并对其进行种内、种间变异分析,barcoding gap检验及NJ聚类图分析,以评估不同序列对千斤拔属植物的物种鉴别能力。结果测序成功率方面,ITS2与rbc L序列对所分析样品的测序成功率为100%,psb A-trn H的测序成功率为85.71%;3条序列中,只有ITS2在barcoding gap检验中具有显著gap;从NJ聚类图来看,ITS2可明显区分千斤拔属植物的不同物种(除F.philippinensis与F.stricta外)。结论 ITS2序列能准确鉴别千斤拔属药用植物,可作为千斤拔药材基原植物鉴定的条形码序列。
Objective To screen a DNA barcode sequence that can accurately and efficiently identify Flemingia Roxb.ex Ait.et Ait.f. medicinal plants. Methods The sequences of ITS2, rbc L and psb A-trn H from 14 medicinal plants were amplified and sequenced. At the same time, the corresponding sequences of Thrips spp. The amplification efficiency of different sequences and the success rate of sequencing were analyzed. The intraspecific and interspecific variation analysis, barcoding gap test and NJ clustering analysis were carried out to evaluate the ability of different sequences to identify the species of Celastrus. Results The sequencing success rate of ITS2 and rbc L sequences was 100% for sequencing samples and 85.71% for sequencing of psb A-trn H. In the 3 sequences, only ITS2 was significant in the barcoding gap test gap; From the NJ cluster diagram, ITS2 clearly distinguishes different species of F. lactiflora (except F.philippinensis and F.stricta). CONCLUSION ITS2 sequence can accurately identify the medicinal plants of Thrips indicus and can be used as a barcode sequence for the identification of Thbc.