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为了探讨发生侧支出芽及重建突触的背根节神经元,其胞体是否也呈现相应的形态变化,以及吗啡对此变化的影响,取10只大鼠分为吗啡备用极组和备用根对照组,切除两组动物脊髓左侧腰段背根及背根节,仅保留L_4背根(备用背根).术后34天灌注固定动物,取L_4根节树脂包埋,等距切取半薄切片和超薄切片.结果发现:两组大鼠术侧背根节体积,与非术侧比较均没有统计学差异;其亮神经元胞体、胞核和核仁的体积也没有显著差异.对照组非术侧亮神经元胞体尼氏体呈细颗粒状;粗面内质网聚集成小簇,分散在胞质中.而术侧尼氏体呈小块状;粗面内质网簇较大,较为密集.吗啡组术侧亮神经元胞质尼氏作也在胞核周围聚集成小块状,有些还呈斑块样.此时可见大的粗面内质网簇,其分布更为密集.这种变化可能与发出有髓传入纤维的亮神经元在脊髓侧支出芽及其重建突触有关.对照组术侧暗神经元胞体、胞核和核仁的体积分别比非术侧显著增大.吗啡组术侧暗神经元上述三种体积又比对照组术侧的大,而且胞质多聚核蛋白体和粗面内质网明显增多、表明与无髓传入纤维在脊髓侧支出芽及重建突触有关的暗神经元胞体也发生了相应的可塑性变化,吗啡对此变化有明显的促进作用.本文首次为研究吗啡促进脊髓可塑性变化提供新的资料,同时提示暗神经元胞体的可塑性
In order to investigate whether there is morphological changes in the synaptic neurons of the dorsal root ganglion and the morphological changes of morphine, 10 rats were divided into morphine spare pole group and control group Group, left lumbar dorsal root and dorsal root ganglion were excised from the two groups, only L 4 dorsal root (spare dorsal root) was reserved.After 34 days of operation, the animals were infused with L 4 root- The results showed that there was no significant difference in the volume of dorsal root ganglion between the two groups, and there was no significant difference in the volume of bright cell neurons, nucleus and nucleolus between the two groups Group of non-operative side bright neurons Nissl body was fine granular; rough endoplasmic reticulum clustered into small clusters, scattered in the cytoplasm, while the operation side Nisshin was small lumps; rough endoplasmic reticulum clusters Large and more dense morphine group of light side of the neuronal cytoplasmic Nissl also gathered around the nucleus into small lumps, and some were also plaque-like.At this time visible large rough endoplasmic reticulum clusters, the distribution of more Is dense.This change may be issued with the myelinated fibers of bright neurons in the spinal cord side of the bud and its reconstruction synapses related to Compared with the non-operative side, the volume of dark cells, the nucleus and the nucleolus in operation group were significantly higher than those in the non-operation side, respectively.The volume of dark neurons in the morphine group was larger than that in the control group, Protein bodies and rough endoplasmic reticulum was significantly increased, indicating that non-pulp fibers into the spinal cord in the sprouting and reconstruction of synaptic dark plastic cell body also occurred corresponding changes in plasticity, morphine significantly promote this change This is the first time to provide new information for studying morphine in promoting the changes of spinal cord plasticity, and at the same time it suggests the plasticity of dark neuronal soma