论文部分内容阅读
为了明确线虫角质层胶原蛋白基因(Col)与根结线虫病害的关系,利用从线虫基因组中预测的Col设计特异引物,克隆了南方根结线虫Meloidogyne incognitacol基因MiCol。该基因完整编码区长903bp,编码300个氨基酸。克隆的MiCol与预测的MiCol基因序列一致性高达99.67%,氨基酸序列一致性高达100%。利用病毒介导的基因沉默技术,将其导入番茄植株并接种南方根结线虫,60 d后,沉默载体pTV-MiColi处理番茄植株的根结数比空载体对照及清水对照分别减少49.4%和49.2%,表明MiCol基因沉默显著降低了南方根结线虫的侵染数量。
In order to clarify the relationship between the nematode cuticular collagen gene (Col) and root knot nematode disease, MiCol gene of Meloidogyne incognitacol was cloned by using Col-specific primers designed from the nematode genome. The complete coding region of this gene is 903bp in length and encodes 300 amino acids. The identity of the cloned MiCol with the predicted MiCol gene was as high as 99.67% and the amino acid sequence identity was as high as 100%. After 60 days, the number of root knots of tomato plants treated with pTV-MiColi silencing vector decreased by 49.4% and 49.2% respectively compared with the control and the control of the water-free medium by using virus-mediated gene silencing technology. %, Indicating that silencing of MiCol gene significantly reduced the number of M. incognita infection.