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为保护野生资源,满足栽培需要,选择全叶延胡索有叶嫩茎为材料,进行了愈伤组织的诱导和分化,不定芽生根,试管苗生根继代增殖、移栽和定植的研究,建立起快速繁殖技术.结果证明:MS+6-BA0.3mg/L+2,4-D2.4mg/L是有叶嫩茎愈伤组织诱导培养和继代增殖培养的理想培养基;MS+ZTO.1+AgN031.0mg/L+IAA0.1mg/L是愈伤组织分化培养的理想培养基;1/3MS+ABT2号0.6mg/L+IAA0.5mg/L是不定芽生根培养和试管苗生根继代增殖培养的理想培养基.无根茎试管苗移栽成活率为83.7%,有根茎试管苗移栽成活率为92.0%,试管苗定植成活率为98.6%,定植的试管苗保持了野生全叶延胡索的所有植物学性状.“,”In order to protect wild resources and meet the need of cultivation,the tender stems with leaves were used as mate- rials to do the research on callus induction and differentiation, rooting and transplanting of tube seedlings and so on, finally estab- lished the rapid propagation system of Corydalis repens. The results showed that the optimum medium for callus induction and sub- culture was MS + 6 - BA0.3 mg/L + 2,4 - D2.4mg/L; MS + ZT0. 1 + AgNO31.0 mg/L + IAA 0.1 mg/L was the ideal medium for callus differentiation and the adventitious buds could be easily induced rooting in the medium of 1/3MS + ABT2 0.6mg/L + IAA0. 5mg/L. The transplanting survival rate of tube seedlings without rhizome was 83.7% and the transplanting survival rate of tube seedlings with rhizome was 92.0%. The stable planting Survival rate was 98.6%. Colonization of plantlets maintained for all biolog- ical traits of Corydalis repens.