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寄生线虫Ditylenchus destructor是许多作物和蔬菜的重要病原物。本研究进行的密封皿内测定结果表明,由枯草芽孢杆菌BL02和G8产生的挥发物能够强烈地抑制D.destructor(24h杀虫活性均达100%)。经挥发物处理后,大部分线虫在1～12h内活动逐渐减弱,24h后完全停止活动。经固相微萃取并经气相色谱-质谱联用技术进一步鉴定,挥发物包括烷烃、醇、酯、酮、酸、胺、肟、酚及杂环类。本研究为微生物活性挥发物的开发和利用提供了有价值的参考并预示了细菌挥发物在未来植物线虫病害生物防治方面的应用潜力。
The parasitic nematode Ditylenchus destructor is an important pathogen for many crops and vegetables. The results of the in-pan assay in this study showed that the volatiles produced by B. subtilis BL02 and G8 strongly inhibited D. destructor (insecticidal activity up to 100% for 24h). After volatiles treatment, most of the nematodes in 1 ~ 12h activity gradually weakened, completely stopped activity after 24h. The compounds were identified by solid-phase microextraction and gas chromatography-mass spectrometry (GC-MS). The volatiles included alkanes, alcohols, esters, ketones, acids, amines, oximes, phenols and heterocycles. This study provides a valuable reference for the development and utilization of microbial active volatiles and predicts the potential of bacterial volatiles in the future biological control of plant nematode diseases.