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应用聚合酶链反应（ＰＣＲ）放大口腔癌组织中Ｈ－ｒａｓ癌基因部分ＤＮＡ顺序，再以特异突变的寡脱氧核苷酸（ＯＤＮ）探针杂交方法检测口腔癌中Ｈ－ｒａｓ癌基因第１２位密码子的Ｇ→Ｔ突变。结果在２１例口腔癌中有６例存在这种突变（２８．５％），而１０例正常门腔粘膜组织中无１例发生突变。提示：Ｈ－ｒａｓ癌基因第１２位密码子→Ｔ突变可能是口腔癌中癌基因激活的方式之一。
The DNA sequence of H-ras oncogene in oral cancer tissues was amplified by polymerase chain reaction (PCR), and the mutation of H-ras oncogene in oral cancer was detected by specific mutated oligodeoxynucleotide (ODN) probe hybridization method G → T mutation of the codon. RESULTS: Sixteen of 21 oral carcinomas had this mutation (28.5%), whereas none of 10 normal mucosal tissues had mutations. Hint: H-ras oncogene codon 12 → T mutation may be one of the ways of oncogene activation in oral cancer.