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目的研究聚裂丛柳珊瑚Rumphella aggregata的化学成分。方法利用硅胶色谱、Sephadex LH-20凝胶色谱、HPLC等手段对化学成分进行分离纯化;通过理化性质、波谱分析方法结合文献对照,鉴定化合物的结构。结果从聚裂丛柳珊瑚甲醇提取物中,共分离鉴定了15个单体化合物:胆甾醇(1)、(22E)-胆甾-5,22-二烯-3β-醇(2)、(22E)-麦角甾-5,22-二烯-3β-醇(3)、麦角甾-5,24(28)-二烯-3β-醇(4)、豆甾-5,24(28)-二烯-3β-醇(5)、柳珊瑚甾醇(6)、胆甾-7-烯-3β,5α,6β-三醇(7)、(22E)-胆甾-7,22-二烯-3β,5α,6β-三醇(8)、麦角甾-7-烯-3β,5α,6β-三醇(9)、(22E)-麦角甾-7,22-二烯-3β,5α,6β-三醇(10)、豆甾-7-烯-3β,5α,6β-三醇(11)、(22E)-豆甾-7,22-二烯-3β,5α,6β-三醇(12)、尿嘧啶(13)、咖啡因(14)、hydratoperidinin(15)。结论化合物1～15均为首次从该属柳珊瑚中分离得到,并首次对化合物hydratoperidinin(15)的1HNMR及13 CNMR信号进行了全归属。化合物12在10μg.mL-1浓度水平,对K562肿瘤细胞株的抑制率为39.17%。
Aim To study the chemical constituents of Rumphella aggregata in Polygalaceae. Methods The chemical constituents were separated and purified by silica gel chromatography, Sephadex LH-20 gel chromatography and HPLC. The structures of the compounds were identified by physico-chemical and spectroscopic methods combined with literature references. Results A total of 15 monomer compounds were isolated and identified from the methanolic extracts of Polygonum cuspidatum L. Cholesterol (1), (22E) -cholesta-5,22-dien-3β- 22E) - ergosta-5,22-dien-3β-ol (3), ergosta-5,24 (28) (6), cholest-7-ene-3β, 5α, 6β-triol (7), (22E) -cholesta-7,22-diene- 3β, 5α, 6β-triol (8), ergosta-7-ene-3β, 5α, 6β- triol (9), (22E) -ergosta-7,22-diene- -triol (10), stigmast-7-ene-3β, 5α, 6β-triol (11), (22E) ), Uracil (13), caffeine (14), hydratoperidinin (15). CONCLUSION Compounds 1-15 were isolated from this genus Salix for the first time and the 1HNMR and 13 CNMR signals of the compound hydratoperidinin (15) were completely assigned. The inhibitory rate of compound 12 to K562 tumor cell line was 39.17% at the concentration of 10μg.mL-1.