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目的：了解我国人群中是否存在ＴＴ病毒（ＴＴＶ）感染，分析国内ＴＴ病毒株基因结构特点。方法：用ＰＣＲ方法检测血清标本中ＴＴＶＤＮＡ，对ＴＴＶＤＮＡ阳性的标本进行序列测定。结果：１０例临床诊断为非甲至非戊型肝炎病人血清标本中，用ＰＣＲ方法检测出５例为ＴＴＶＤＮＡ阳性。将其中的一株命名为ＴＴＶＣＨ１（ＴＴＶ中国株１）并进行序列测定，该序列与日本ＴＴＶ部分基因（ＣＬＯＮ２２）相对应位置的核苷酸同源性为９８％。用ＢＬＡＳＴ程序检索了ＧｅｎＢａｎｋ＋ＥＭＢＬ＋ＰＤＢ序列库的３３２９４９个序列数据，没有发现与之有明显同源性的序列；与已知的肝炎病毒（Ａ至Ｇ）也没有明显的相关性，而只同日本报道的部分基因有很高的同源性。结论：该研究建立了检测ＴＴＶ的ＰＣＲ方法并测定了ＴＴＶ中国株的部分基因序列，首次证实在我国人群中存在ＴＴＶ的感染。
Objective: To understand whether there is a TTV infection in our population and to analyze the structural characteristics of domestic TT virus strains. Methods: TTVDNA was detected by PCR in serum samples, and the sequence of TTVDNA positive samples was determined. Results: Ten cases were clinically diagnosed as non-A to non-E hepatitis, and 5 cases were positive for TTVDNA by PCR. One of the strains was named TTVCH1 (TTV China strain 1) and was sequenced. The nucleotide sequence homology was 98% with the corresponding position of the Japanese TTV partial gene (CLON22). 332949 sequence data of GenBank + EMBL + PDB sequence library were retrieved by BLAST program and no sequence with obvious homology was found. There was no obvious correlation with the known hepatitis virus (A to G), but only with the reported Some genes have high homology. Conclusion: This study established a PCR method for the detection of TTV and determined the partial gene sequence of the Chinese strain of TTV, which for the first time confirmed the presence of TTV infection in our population.