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对罗丹明B荧光猝灭法测定饮用水中溴酸根的含量进行了研究。考察了酸度、罗丹明B的浓度、反应时间和温度等对测定结果的影响,得到了最优化的实验条件:最大激发波长为557nm,最大发射波长为582nm,反应介质为3.00mL 2mol/L HCl,罗丹明B浓度为1×10~(-4) mol/L,反应时间为30min,反应温度为70℃。在优化的实验条件下,溴酸根在0—2.0μg/25mL之间存在线性关系,相关系数为0.9979,检出限为2.08ng/mL,将该方法用于饮用水中溴酸根的测定,样品加标回收率为85%—115%。
The determination of bromate in drinking water by fluorescence quenching of rhodamine B was studied. The effects of acidity, rhodamine B concentration, reaction time and temperature on the determination results were investigated. The optimized experimental conditions were obtained: the maximum excitation wavelength was 557nm, the maximum emission wavelength was 582nm, the reaction medium was 3.00mL 2mol / L HCl , Rhodamine B concentration of 1 × 10 -4 mol / L, reaction time of 30 min and reaction temperature of 70 ℃. Under the optimal experimental conditions, there was a linear relationship between bromate concentration of 0-2.0μg / 25mL with a correlation coefficient of 0.9979 and a detection limit of 2.08ng / mL. The method was applied to the determination of bromate in drinking water. Spike recovery was 85% -115%.