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目的 研究肝细胞低密度脂蛋白受体，胆汁中胆汁酸和胆固醇的动态变化及其在胆囊结石过程中可能的机制。方法 利用高胆固醇膳食（ｈｉｇｈ ｃｈｏｌｅｓｔｅｒｏｌｄｉｅｔ，ＨＣＤ） 诱发兔胆囊胆固醇结石模型，分别对进食高胆固醇膳食后１ 、２、３ 、４ 周组及对照组，采用双波长薄层扫描法测定胆汁中胆汁酸，硫磷铁法测定胆汁中胆固醇以及１２５ＩＬＤＬ放免标记法测定肝细胞低密度脂蛋白受体（ＬＤＬｒ）活性。结果 高胆固醇膳食后１ 周开始出现胆囊结石，２ 、３ 和４ 周组分别有４／１０，６／１０ 和７／１０ 例出现胆囊结石；胆汁中甘氨脱氧胆酸（ＧＤＣＡ） 和甘氨胆酸（ＧＣＡ） ，前者１ 周时明显升高，以后逐渐降低，但差异无显著性（ Ｐ＞０ ．０５），后者逐渐降低，以３ 和４ 周组明显（ Ｐ＜ ０．０５） ；胆汁胆固醇随着ＨＣＤ进食时间延长呈进行性升高，２ 、３ 、４ 周组与对照组比较差异有显著性（ Ｐ＜ ０ ．０５） ，３ 和４ 周组升高最明显，１２５ＩＬＤＬ 与ＬＤＬｒ 的最大结合力Ｂｍａｘ 在１ 周组略升高（ Ｐ＞ ０ ．０５） ，２ 周组逐渐下降，３ 和４ 周组时明显下降（Ｐ＜ ０．０５），解离常数（Ｋｄ） 值逐渐升高，以３ 和４ 周组明显（Ｐ＜ ０ ．０５） 。结?
Objective To study the dynamic changes of hepatocyte low density lipoprotein receptor, bile acid and cholesterol in bile and its possible mechanism in the gallstone process. Methods Cholesterol gallstone model was induced by high cholesterol diet (HCD) in rabbits. The levels of bile acid (bile acid) in bile were determined by dual-wavelength thin-layer scanning after 1, 2, , Determination of bile cholesterol by the method of iron phosphorus and 125I LDL radioimmunoassay determination of low density lipoprotein receptor (LDLr) activity of liver cells. Results Gallbladder stones appeared in the first week after high cholesterol diet. Gallstone was found in 4, 10, 6/10, and 7/10 cases in groups 2, 3, and 4 weeks respectively. GDC and GDAM in bile (P <0.05), while the latter decreased gradually (P <0.05), and the former was significantly increased at 1 week and then gradually decreased, but the difference was not significant (P> 0.05) ; Bile cholesterol increased progressively with the prolonged HCD feeding, the difference was significant at 2, 3 and 4 weeks (P <0 .05), and at 3 and 4 weeks, the level of 125 I -LDL and The maximal binding capacity of LDLr Bmax increased slightly in the first week (P> 0.05), decreased gradually in the second week, decreased significantly in the third and fourth week (P <0.05), dissociation constant (Kd) Gradually increased to 3 and 4 weeks group was significantly (P <0 .05). Knot?