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目的 探讨早产母初乳免疫物质的含量 ,为早产儿早期母乳喂养提供理论依据。 方法 用流式细胞仪直接和间接免疫荧光标记法测定早产母初乳中表达 CD3、CD4、CD8、CD1 9分化群的细胞比例 ,用双抗体夹心酶联免疫吸附法 (EL ISA)测定初乳中肿瘤坏死因子 -α(TNF-α)的含量 ,并与正常足月初乳组对比分析。 结果 早产母初乳中 T3、T4、T8淋巴细胞比例及 T4/ T8比值稍低于足月组 ,B淋巴细胞比例稍高于足月组 ,但上述结果两组间差异均无显著性 (P>0 .0 5 )。早产母初乳中TNF- α的含量高于足月组 ,其中位数分别为 376 .96 g/ ml,111.38pg/ ml,二者间差异有显著性 (P<0 .0 5 )。 结论 早产母初乳中含有丰富的免疫活性物质 ,对早产儿更应提倡母乳喂养、早哺母乳。
Objective To investigate the content of immune substances in preterm labor colostrum and provide theoretical basis for early breastfeeding. Methods The ratio of CD3, CD4, CD8 and CD19-expressing cells in preterm primordial mother’s milk was determined by flow cytometry, direct and indirect immunofluorescent labeling, and the colostrum was detected by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) Tumor necrosis factor-α (TNF-α) levels, and compared with normal full-term colostrum group analysis. Results The proportions of T3, T4 and T8 lymphocytes and the ratio of T4 / T8 in preterm primordial colostrums were slightly lower than those in term groups. The proportion of B lymphocytes in preterm labor was slightly higher than that in term groups. However, there was no significant difference between the two groups > 0 .0 5). The content of TNF- α in preterm labor colostrum was higher than that in term group, with the median of 376.96 g / ml and 111.38 pg / ml, respectively. There was a significant difference between the two groups (P <0.05). Conclusion Premature mother colostrum is rich in immunocompetent substances, should promote breastfeeding premature children, breastfeeding early.