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目的:以表皮生长因子(epidermal growth factor,EGF)刺激MDA-MB-231乳腺癌细胞胞体和伪足分离,在此基础上,鉴定Rab基因家族在胞体和伪足中的差异表达谱。方法:运用Boyden小室技术分离EGF刺激下MDA-MB-231乳腺癌细胞的胞体和伪足,并鉴定Rab基因家族在乳腺癌细胞胞体和伪足中的差异表达谱。结果:RAB1A等21个基因在胞体中高表达,RAB7A等16个基因在伪足中高表达,RAB3B等24个基因在胞体和伪足中的表达量大体相等。结论 :本研究建立的基于Boyden小室技术的实验方法可以有效分离乳腺癌细胞的胞体和伪足,在此基础上鉴定了一类在EGF趋化刺激下乳腺癌细胞伪足中富集的Rab基因,从而为进一步深入研究乳腺癌细胞伪足伸展的确切分子机制提供新的思路和研究方向。
OBJECTIVE: To separate the somatic and pseudopodia of MDA-MB-231 breast cancer cells with epidermal growth factor (EGF), and to identify the differential expression profiles of Rab gene family in soma and pseudopod. Methods: Boyden chamber technique was used to separate the somatic cells and pseudopodia of MDA-MB-231 breast cancer cells stimulated by EGF and to identify the differential expression profiles of Rab gene family in somatic cells and pseudopodia of breast cancer cells. RESULTS: Twenty-one genes, including RAB1A, were highly expressed in the soma. 16 genes such as RAB7A were highly expressed in pseudopods. The expression of 24 genes such as RAB3B in the somatic and pseudopodia were almost the same. CONCLUSION: The experimental method based on Boyden chamber technique established in this study can effectively separate the soma and pseudopodia of breast cancer cells. Based on this, a group of Rab genes enriched in the pseudopodia of breast cancer cells stimulated by EGF chemotaxis were identified , Which will provide new ideas and research direction for the further study of the exact molecular mechanism of pseudopodal extension of breast cancer cells.