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为探索乙肝病毒 (HBV)S基因 (HBVS)亚型、前C区点突变与血清乙肝病毒e(HBe)系统之间的关系 ,我们对98例血清学确诊且HBVDNA阳性的乙型肝炎患者血清采用 3′端特异性引物聚合酶链反应 (3′BS PCR)进行了1896位核苷酸的检测分析 ,同时进行片段长度多态性分析 (PCR RFLP)检测HBV亚型。结果显示 :① 98例患者中 6 3例有突变株感染 (6 4.3% ) ,野生株和突变株混合感染的检出率在HBeAg阳性组为 5 7.8% ,抗 HBe阳性组中为34 .7% ,两组间有显著性差异 (P <0 .0 5 ) ;② 98例患者中HBVS亚型Ⅰ 6 8例 (6 9.4% ) ,其中HBeAg阳性与抗HBe阳性分别为 31与 32例 ;③HBVS亚型Ⅰ与Ⅱ突变株检出率分别为 80 .9%与 2 4.0 % ,两者有极显著差异 (P <0 .0 1) ,亚型Ⅰ中野生株和突变株混合感染率为 5 7.4%。结果表明 :HBVS亚型与HBe系统无必然的因果关系 ,但HBe系统与前C区第 1896位点突变有关。HBV前C区第 1896位点突变与HBVS亚型有关 ,以亚型Ⅰ易发生。变异株的产生可能受HBVS亚型和机体免疫压力所致。
In order to explore the relationship between HBV S subtype, pre-C point mutation and serum hepatitis B e (HBe) system, we analyzed 98 cases of serologically confirmed HBVDNA-positive patients with hepatitis B The 3’-end PCR (3’BS PCR) was used to detect the 1896 nucleotides, and the PCR-RFLP was used to detect the HBV subtype. The results showed that: (1) of the 98 patients, 63 cases were infected with the mutant strain (6 4.3%). The prevalence of mixed infection in wild and mutant strains was 57.8% in HBeAg positive group and 34.7 in HBeAg positive group %, There was significant difference between the two groups (P <0.05). ② The HBV subtype Ⅰ 68 cases (6 9.4%) in 98 patients, of which 31 and 32 were HBeAg positive and anti HBe positive respectively; (3) The detection rates of HBsAg subtypes Ⅰ and Ⅱ were 80.9% and 2.04%, respectively, with significant difference (P0.01). The mixed infection rate of wild type and mutant strains in subtype Ⅰ was 5 7.4%. The results showed that there was no necessary causal relationship between HBV subtypes and HBe system, but the HBe system was associated with mutation at 1896 in pre-C region. HBV pre-C region 1896 site mutations associated with HBVS subtypes to subtype Ⅰ prone. Mutant strains may be caused by HBV subtype and immune pressure.